B Lymphocytes and Macrophages in the Perivascular Adipose Tissue Are Associated With Coronary Atherosclerosis: An Autopsy Study

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Farias-Itao, Daniela Souza ^[1] ; Pasqualucci, Carlos Augusto ^[1] ; Nishizawa, Aline ^[1] ; da Silva, Luiz Fernando Ferraz ^[1] ; Campos, Fernanda Marinho ^[1] ; Bittencourt, Marcio Sommer ^{[2, 3, 4, 5]} ; da Silva, Karen Cristina Souza ^[1] ; Leite, Renata Elaine Paraizo ^{[1, 6]} ; Grinberg, Lea Tenenholz ^{[7, 1]} ; Ferretti-Rebustini, Renata Eloah de Lucena ^{[8, 1]} ; Jacob-Filho, Wilson ^{[1, 6]} ; Suemoto, Claudia Kimie ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Med Sch, Dept Pathol, Sao Paulo - Brazil

^[2] Hosp Israelita Albert Einstein, Prevent Med Ctr, Sao Paulo - Brazil

^[3] Hosp Israelita Albert Einstein, Cardiol Program, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Univ Hosp, Ctr Clin & Epidemiol Res, BR-05508 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Univ Hosp, Div Internal Med, BR-05508 Sao Paulo - Brazil

^[6] Univ Sao Paulo, Med Sch, Discipline Geriatr, Sao Paulo - Brazil

^[7] Univ Calif San Francisco, Dept Neurol, Memory & Aging Ctr, San Francisco, CA - USA

^[8] Univ Sao Paulo, Sch Nursing, Med Surg Nursing Dept, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE AMERICAN HEART ASSOCIATION; v. 8, n. 24 DEC 17 2019.
• Citações Web of Science: 0

Resumo

Background Macrophages and T lymphocytes in the perivascular adipose tissue (PvAT) were previously linked to coronary artery disease. However, the role of these cells and B lymphocytes in the human PvAT adjacent to unstable atherosclerotic plaques has not been investigated. Moreover, previous studies were inconclusive on whether PvAT inflammation was restricted to the surroundings of the atheroma plaque. Methods and Results Coronary arteries were freshly dissected with the surrounding PvAT. Atherosclerotic plaques were classified according to the internationally accepted anatomopathological criteria. Immune cells in the PvAT were detected using immunohistochemistry and then quantified. We used linear and logistic regressions with robust standard errors, adjusted for possible confounding factors. In 246 atherosclerotic plaques (205 stable and 41 unstable plaques) from 82 participants (mean age=69.0 +/- 14.4 years; 50% men), the percentage of arterial obstruction was positively correlated with the densities of CD68(+) macrophages (P=0.003) and CD20(+) B lymphocytes (P=0.03) in the periplaque PvAT. The number of cells was greater in the periplaque PvAT than in the distal PvAT (macrophages, P<0.001; B lymphocytes, P=0.04). In addition, the density of macrophages in the periplaque PvAT was greater in the presence of unstable plaques (P=0.03) and was also greater near unstable plaques than in the distal PvAT (P=0.001). CD3(+) T lymphocytes were not associated with percentage of obstruction and stable/unstable plaque composition. Conclusions The density of CD20(+) B lymphocytes and CD68(+) macrophages in periplaque PvAT was increased with plaque size, and the CD68(+) macrophages were greater near unstable atherosclerotic plaques than near stable lesions. This inflammation was more intense in the periplaque PvAT than in the PvAT distal to the atherosclerotic plaques. (AU)

• Processo FAPESP: 13/00335-2 - Associação entre inflamação no tecido adiposo epicárdico e doença arterial coronariana: um estudo clínico patológico
• Beneficiário: Claudia Kimie Suemoto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/12290-3 - Associação entre inflamação no tecido adiposo epicárdico e doença arterial coronariana: um estudo clinicopatológico
• Beneficiário: Daniela Souza Farias Itao
• Modalidade de apoio: Bolsas no Brasil - Mestrado