Zinc uptake from ZnSO4 (aq) and Zn-EDTA ((aq)) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy

Autor(es)

Montanha, Gabriel S. ^[1] ; Rodrigues, Eduardo S. ^[1] ; Romeu, Sara L. Z. ^[1] ; de Almeida, Eduardo ^[1] ; Reis, Andre R. ^[2] ; Lavres Jr, Jose ; Pereira de Carvalho, Hudson W. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Ave Centenario 303, BR-13416000 Sao Paulo, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Rua Domingos da Costa Lopes 780, BR-17602496 Tupa, SP - Brazil

^[3] Lavres Jr, Jr., Jose, Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Ave Centenario 303, BR-13416000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Science; v. 292, MAR 2020.
• Citações Web of Science: 1

Resumo

This study investigated the dynamic of zinc (Zn) uptake and the root-to-shoot Zn-transport when supplied as ZnSO4 ((aq)) or Zn-EDTA ((aq)) in soybean seedlings using in vivo X-ray fluorescence (XRF) and X-ray absorption spectroscopy (XANES). The time-resolved X-ray fluorescence showed that plants absorbed ca. 10-fold more Zn from ZnSO4 ((aq)) than from Zn-EDTA ((aq)). However, the uptake velocity did not influence the amount of Zn in the stem. It let furthermore appear that the plants were able to reduce the absorption of Zn from Zn-EDTA ((aq)) earlier than ZnSO4 ((aq)). Thus, the entrance of Zn2+ into the roots is not necessarily accompanied by SO4(aq)2- Regardless the source, the Zn distribution and its transport in the stem were spatially correlated to the bundles and cortex nearby the epidermal cells. Its chemical speciation showed that Zn is neither transported as ZnSO4 ((aq)) nor as Zn-EDTA ((aq)), indicating that these compounds are retained in the roots or biotransformed on in the root-solution interface. Zn2+ was long-distance transported complexed by organic molecules such as histidine, malate, and citrate, and the proportion of ligands was affected by the concentration of Zn2+ in the stem rather than by the type of Zn source. (AU)

• Processo FAPESP: 15/19121-8 - EMU concedido no processo 2015/05942-0: sistema de microfluorescência de Raios X
• Beneficiário: Hudson Wallace Pereira de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 15/05942-0 - Investigação do metabolismo de nanomateriais absorvidos por plantas empregando-se espectroscopias vibracional e de Raios X
• Beneficiário: Hudson Wallace Pereira de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/16375-4 - Efeitos da aplicação foliar de La e Ce em plantas de soja
• Beneficiário: Eduardo Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado