Uterine cervix as a fundamental part of the pathogenesis of pregnancy loss associated with ascending placentitis in mares

Autor(es)

Fernandes, C. B. ^{[1, 2]} ; Ball, B. A. ^[2] ; Loux, S. C. ^[2] ; Boakari, Y. L. ^[2] ; Scoggin, K. E. ^[2] ; El-Sheikh Ali, H. ^{[2, 3]} ; Cogliati, B. ^[4] ; Esteller-Vico, A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, BR-05508270 Sao Paulo - Brazil

^[2] Univ Kentucky, Gluck Equine Res Ctr, Lexington, KY 40546 - USA

^[3] Univ Mansoura, Fac Vet Med, Theriogenol Dept, Mansoura 35516 - Egypt

^[4] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 145, p. 167-175, MAR 15 2020.
• Citações Web of Science: 1

Resumo

Anatomical and molecular changes in the cervical barrier in women are a fundamental part of the pathogenesis of pregnancy loss associated with chorioamnionitis. However, there is little information regarding changes in the cervix associated with ascending infection in pregnant mares. To better characterize morphological and molecular changes in the cervix during placentitis, we examined full thickness histology and mRNA expression for a number of inflammatory and endocrine factors in the mucosa and stroma of the cervix of mares (n = 5) after experimental induction of placentitis via transcervical inoculation with Streptococcus equi ssp zooepidemicus at approximately 290d of gestation. Gestationally age-matched mares (n = 4) served as controls. Target transcripts included steroid receptors (PGR, ESR1 and 2), OXTR, prostaglandins synthases and receptors (PTGS1, PTGS2, PGES, PGFS, PTGER2 and PTGER4), cytokines (IL1b, IL6, CLCX8, IL10 and TNF alpha) and acute phase proteins (SAA). Histologically, a marked modification in the cervical epithelia and stroma was characterizing cervicitis. Additionally, the mRNA expression of IL1 beta, IL6, CXCL8, SAA and PTGS2 was greater (P < 0.05) in both mucosa and stroma of the inoculated mares; whereas TNF alpha, IL10 and PGES were upregulated (P < 0.05) only in the cervical mucosa. Progesterone receptor, ESR1 and PTGER4 were upregulated in the cervical stroma of placentitis mares. In conclusion, the cervical response to placentitis was characterized by an upregulation of inflammatory cytokines that was accompanied by induction of PTGS2 and PGES. Further, receptors known to be associated with relaxation of the cervix in other species (ESR1 and PTGER4) were upregulated in the cervical stroma of placentitis mares. These findings indicate that the cervix is not only a physical barrier but that it has an active role in the pathogenesis of ascending placentitis. (C) 2019 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 15/00049-5 - Uso de microRNAs placentários como biomarcadores de placentite
• Beneficiário: Claudia Barbosa Fernandes
• Modalidade de apoio: Bolsas no Exterior - Pesquisa