In vitro grafting of Psidium guajava in Psidium cattleianum for the Management of the Meloidogyne enterolobii

Autor(es)

Fortuna Macan, Giovana Prado ^{[1, 2]} ; Cardoso, Jean Carlos ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Ctr Ciencias Agr, Araras - Brazil

^[2] Univ Politecn Valencia, Escuela Tecn Super Ingn Agron & Medio Nat, Valencia - Spain

^[3] Univ Fed Sao Carlos, Lab Plant Physiol & Tissue Culture, Dept Biotechnol Plant & Anim Prod, Ctr Ciencias Agr, Araras - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF FRUIT SCIENCE; v. 20, n. 1, p. 106-116, JAN 2 2020.
• Citações Web of Science: 0

Resumo

Guava is one of the most important tropical fruit crops; however, its cultivation has been affected by the prevalence of the nematode Meloidogyne enterolobii, which leads to a large-scale reduction in the production of fruits and restricts new plantations in the contaminated areas. The discovery of the resistance of Psidium cattleianum to this nematode has increased the possibility of its selective use as rootstock for guava; a few studies have worked on the graft; however, the rootstock exhibits a high incompatibility with the commercial varieties of guava. Therefore, the present study aimed to develop an in vitro-grafting technique of guava scions under rootstocks of P. cattleianum obtained using an in vitro seed germination method. The successful in vitro-grafting was performed using semisolid MS1/2 (Murashige and Skoog) culture medium with 0.5 mg L-1 of Benzyladenine. P. cattleianum with approximately 3 cm length was used as rootstocks, and apical guava stems with 0.5 cm length were used as scions. The grafting procedure used was wedge grafting. To increase the success rate of in vitro-grafting, we used a sterile paper moistened with sterilized distilled water to prevent dehydration. The use of MS1/2 and the wedge grafting resulted in the overall success rate of 30% for the graft between P. cattleianum and Psidium guajava. In conclusion, this is the first report of successful in vitro-grafting between guava and P. cattleianum. (AU)

• Processo FAPESP: 16/07571-1 - Obtenção de híbridos interespecíficos entre a goiaba (Psidium guajava L.) e o araça (Psidium cattleianum) a partir da técnica de cultura
• Beneficiário: Giovana Prado Fortuna Macan
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/07094-0 - Utilização de ferramentas biotecnológicas em goiabeira (Psidium guajava L.) visando a resistência a Meloidogyne enterolobii
• Beneficiário: Jean Carlos Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular