Physical exercise increases ROCK activity in the skeletal muscle of middle-aged rats

Autor(es)

Munoz, Vitor Rosetto ^[1] ; Gaspar, Rafael Calais ^[1] ; Esteca, Marcos Vinicius ^[2] ; Baptista, Igor Luchini ^[2] ; Lins Vieira, Renan Fudoli ^[1] ; Ramos da Silva, Adelino Sanchez ^{[3, 4]} ; de Moura, Leandro Pereira ^{[1, 5]} ; Cintra, Dennys Esper ^{[3, 6]} ; Ropelle, Eduardo Rochete ^{[1, 5]} ; Pauli, Jose Rodrigo ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Lab Mol Biol Exercise, Limeira, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Lab Cellular & Tissue Biol, Fac Appl Sci, Limeira - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, UNICAMP, OCRC, Campinas, SP - Brazil

^[6] Univ Estadual Campinas, UNICAMP, Lab Nutr Genom, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MECHANISMS OF AGEING AND DEVELOPMENT; v. 186, MAR 2020.
• Citações Web of Science: 0

Resumo

The physical exercise is a potential strategy to control age-related metabolic disorders, such as insulin resistance, impaired glucose homeostasis, and type 2 diabetes. Rho-kinase (ROCK) increases skeletal muscle glucose uptake through Insulin Receptor Substrate 1 (IRS1) phosphorylation. Here, we investigated the role of physical exercise in ROCK pathway in the skeletal muscle of Fischer middle-aged rats. Firstly, we observed the ROCK distribution in different skeletal muscle fiber types. ROCK signaling pathway (ROCK1 and ROCK2) and activity (pMYPT1) were higher in the soleus, which was associated with increased insulin signaling pathway (pIR, pIRS1, pPDK, pGSK3 beta). Middle-aged rats submitted to physical exercise, showed the upregulation of ROCK2 content and normalized RhoA (ROCK activator enzyme) levels in soleus muscle compared with middle-aged sedentary rats. These molecular changes in middle-aged exercised rats were accompanied by higher insulin signaling (pIRS1, pGSK3 beta, pAS160, GLUT4) in the soleus muscle. Reinforcing these findings, when pharmacological inhibition of ROCK activity was performed (using Y-27632), the insulin signaling pathway and glucose metabolism-related genes (Tpi, Pgk1, Pgam2, Eno3) were decreased in the soleus muscle of exercised rats. In summary, ROCK signaling seems to contribute with whole-body glucose homeostasis (similar to 50 %) through its higher upregulation in the soleus muscle in middle-aged exercised rats. (AU)

• Processo FAPESP: 16/18488-8 - O papel do exercício físico nas vias moleculares de captação de glicose em camundongos nocautes para GLUT-4
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/26000-2 - O papel da proteína Rock sobre a captação de glicose no músculo esquelético de roedores exercitados durante o processo de envelhecimento
• Beneficiário: Vitor Rosetto Muñoz
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 18/20872-6 - Efeitos do exercício físico sobre a via de sinalização da proteína Rock e regulação da lipogênese e gliconeogênese no fígado de ratos envelhecidos
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular