The Effects of Aging on Rho-Kinase and Insulin Signaling in Skeletal Muscle and White Adipose Tissue of Rats

Autor(es)

Munoz, Vitor Rosetto ^[1] ; Gaspar, Rafael Calais ^[1] ; Kuga, Gabriel Keine ^[1] ; Betim Pavan, Isadora Carolina ^[2] ; Simabuco, Fernando Moreira ^[2] ; Ramos da Silva, Adelino Sanchez ^{[3, 4]} ; de Moura, Leandro Pereira ^{[1, 5, 6, 7]} ; Cintra, Dennys Esper ^{[5, 8]} ; Ropelle, Eduardo Rochete ^{[1, 5, 7]} ; Pauli, Jose Rodrigo ^{[1, 5, 7]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Lab Mol Biol Exercise, UNICAMP, Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Sch Appl Sci FCA, Lab Funct Properties Foods, UNICAMP, Limeira, SP - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, OCRC, UNICAMP, Campinas, SP - Brazil

^[6] Sao Paulo State Univ, UNESP, Postgrad Program Motor Sci, Sao Paulo, SP - Brazil

^[7] Univ Estadual Campinas, CEPECE Ctr Res Sport Sci, UNICAMP, Sch Appl Sci, Limeira, SP - Brazil

^[8] Univ Estadual Campinas, Lab Nutr Genom, UNICAMP, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES; v. 75, n. 3, p. 432-436, MAR 2020.
• Citações Web of Science: 4

Resumo

The insulin receptor substrate 1 regulates insulin-mediated glucose uptake and is a target of Rho-kinase (Rock); however, the relationship between age-related insulin resistance and Rock signaling specifically in skeletal muscle and adipose tissue is unknown. We evaluated the content and activity of Rock in C2C12 myotubes, and in skeletal muscle and white adipose tissue (WAT) from two rodent models that differ in their patterns of body fat accumulation during aging (Wistar and Fischer 344 rats). Body fat gain in the Wistar rats was greater than in Fischer rats and only Wistar rats had impairment of whole-body insulin sensitivity. Rock activity and insulin signaling were impaired in skeletal muscle in both rat models, but only middle-aged Wistar rats had higher Rock activity in WAT. These data are consistent with a positive role of Rock in regulating insulin signaling in both skeletal muscle and its negative role in the adipose tissue, suggesting that Rock activity in adipose tissue is important in age-related insulin resistance. (AU)

• Processo FAPESP: 12/13558-7 - Caracterização molecular das proteínas S6Ks na obesidade e em suas doenças associadas
• Beneficiário: Fernando Moreira Simabuco
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/18488-8 - O papel do exercício físico nas vias moleculares de captação de glicose em camundongos nocautes para GLUT-4
• Beneficiário: José Rodrigo Pauli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/26000-2 - O papel da proteína Rock sobre a captação de glicose no músculo esquelético de roedores exercitados durante o processo de envelhecimento
• Beneficiário: Vitor Rosetto Muñoz
• Modalidade de apoio: Bolsas no Brasil - Mestrado