The Effects of Aging on Rho-Kinase and Insulin Signaling in Skeletal Muscle and White Adipose Tissue of Rats

Author(s)

Munoz, Vitor Rosetto ^[1] ; Gaspar, Rafael Calais ^[1] ; Kuga, Gabriel Keine ^[1] ; Betim Pavan, Isadora Carolina ^[2] ; Simabuco, Fernando Moreira ^[2] ; Ramos da Silva, Adelino Sanchez ^{[3, 4]} ; de Moura, Leandro Pereira ^{[1, 5, 6, 7]} ; Cintra, Dennys Esper ^{[5, 8]} ; Ropelle, Eduardo Rochete ^{[1, 5, 7]} ; Pauli, Jose Rodrigo ^{[1, 5, 7]}

Affiliation

^[1] Univ Estadual Campinas, Lab Mol Biol Exercise, UNICAMP, Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Sch Appl Sci FCA, Lab Funct Properties Foods, UNICAMP, Limeira, SP - Brazil

^[3] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, OCRC, UNICAMP, Campinas, SP - Brazil

^[6] Sao Paulo State Univ, UNESP, Postgrad Program Motor Sci, Sao Paulo, SP - Brazil

^[7] Univ Estadual Campinas, CEPECE Ctr Res Sport Sci, UNICAMP, Sch Appl Sci, Limeira, SP - Brazil

^[8] Univ Estadual Campinas, Lab Nutr Genom, UNICAMP, Limeira, SP - Brazil

• Document type: Journal article
• Source: JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES; v. 75, n. 3, p. 432-436, MAR 2020.
• Web of Science Citations: 4

Abstract

The insulin receptor substrate 1 regulates insulin-mediated glucose uptake and is a target of Rho-kinase (Rock); however, the relationship between age-related insulin resistance and Rock signaling specifically in skeletal muscle and adipose tissue is unknown. We evaluated the content and activity of Rock in C2C12 myotubes, and in skeletal muscle and white adipose tissue (WAT) from two rodent models that differ in their patterns of body fat accumulation during aging (Wistar and Fischer 344 rats). Body fat gain in the Wistar rats was greater than in Fischer rats and only Wistar rats had impairment of whole-body insulin sensitivity. Rock activity and insulin signaling were impaired in skeletal muscle in both rat models, but only middle-aged Wistar rats had higher Rock activity in WAT. These data are consistent with a positive role of Rock in regulating insulin signaling in both skeletal muscle and its negative role in the adipose tissue, suggesting that Rock activity in adipose tissue is important in age-related insulin resistance. (AU)

• FAPESP's process: 12/13558-7 - Molecular characterization of S6Ks in obesity and its associated diseases
• Grantee: Fernando Moreira Simabuco
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/18488-8 - The role of physical exercise on molecular pathways of glucose uptake in GLUT-4 knockout mice
• Grantee: José Rodrigo Pauli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/26000-2 - The role of Rock protein on glucose uptake in the skeletal muscle of exercised rodents during the aging process
• Grantee: Vitor Rosetto Muñoz
• Support Opportunities: Scholarships in Brazil - Master