ROLE OF PHYSICAL EXERCISE IN REGULATION OF ROCK PROTEIN IN OBESE MICE: EFFECTS ON INSULIN SIGNALLING AND GLUCOSE HOMEOSTASIS

• Grant number: 13/00554-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: José Rodrigo Pauli
• Grantee: Leandro Pereira de Moura
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated scholarship(s): 13/14149-6 - Physiological role of ROCK1 in glucose homeostasis, BE.EP.DR

Abstract

Obesity is a disease that is growing at an alarming rate and is associated with insulin resistance and development of chronic diseases such as cardiovascular diseases, some cancers and type 2 diabetes mellitus. Therefore, understanding the molecular mechanisms related to insulin signaling becomes essential to reduce to or reverse the progression of this alarming epidemiological reality. Insulin resistance can be installed in muscle and also in hepatic tissue, contributing substantially to initially alteration in postprandial glycaemia and later in fasting glycaemia. Moreover, the regular practice of physical exercise can improve insulin action in skeletal muscle increasing glucose uptake and in the liver reducing the formation of new glucose (gluconeogenesis). However, the effects of physical exercise on the insulin molecular pathway are not very well known. Recently, a new protein has been classified as a mediator of insulin signaling by enhancing its effects, this new protein is Rho-kinase (Rock) that acts on peripheral tissues (skeletal muscle, hepatic and adipose tissues) and in the hypothalamus, contributing to 50% insulin action in the intracellular environment. On the other hand, experimental animals that had the deletion or inhibition of Rock became insulin resistant. Seen this, studies about this new protein are necessary to better understand this complex signaling. Up to now no one study was performed to evaluate the physical exercise effects on protein expression of Rock, this project aims to investigate the role of physical exercise in acute and chronic regulation of protein Rock and its effects on signaling insulin in liver and skeletal muscle of obese mice.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOURA, LEANDRO P.; MUNOZ, VITOR R.; LIMA, INES S.; GASPAR, RAFAEL C.; VEIGA, CAMILLA B.; ROPELLE, EDUARDO R.; CINTRA, DENNYS E.; SILVA, ADELINO S. R.; BOTEZELLI, JOSE DIEGO; MEKARY, RANIA A.; et al. Physical Exercise Increases Glucose Uptake in Skeletal Muscle of Obese Mice Through Rho-Kinase Metabolism. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, v. 48, n. 5, p. 1-pg., 2016-05-01. (13/21491-2, 13/00554-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MOURA, Leandro Pereira de. Role of physical exercise in regulation of rock protein in obese mice: effects on insulin signalling and glucose homestasis. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Rio Claro Rio Claro.