Autor(es)

de Carli, Gabriel Jose ^{[1, 2]} ; Rotela, Abdon Troche ^{[3, 4]} ; Lubini, Greice ^[1] ; Contiliani, Danyel Fernandes ^{[1, 2]} ; Candia, Nidia Benitez ^[3] ; Depintor, Thiago S. ^[2] ; Pinto de Abreu, Fabiano Carlos ^{[1, 2]} ; Paulino Simoes, Zila Luz ^{[1, 2]} ; Rios, Danilo Fernandez ^[3] ; Pereira, Tiago Campos ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[3] Univ Nacl Asuncion, Fac Ciencias Exactas & Nat, San Lorenzo - Paraguay

^[4] Univ Nacl Asuncion, Fac Politecn, San Lorenzo - Paraguay

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 1 2020.
• Citações Web of Science: 0

Resumo

RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development of therapeutic strategies and generation of biotechnological products. Here we present a free software tool for the rational design of RNAi effectors, named siRNA and shRNA designer (SSD). SSD incorporates our previously developed software Strand Analysis to construct template DNAs amenable for the large scale production of mono-, bi- and trivalent multimeric shRNAs, via in vitro rolling circle transcription. We tested SSD by creating a trivalent multimeric shRNA against the vitellogenin gene of Apis mellifera. RT-qPCR analysis revealed that our molecule promoted a decrease in more than 50% of the target mRNA, in a dose-dependent manner, when compared to the control group. Thus, SSD software allows the easy design of multimeric shRNAs, for single or multiple simultaneous knockdowns, which is especially interesting for studies involving large amounts of double-stranded molecules. (AU)

• Processo FAPESP: 16/06657-0 - Análise do sistema endócrino e do ciclo circadiano como moduladores do desenvolvimento de Apis mellifera
• Beneficiário: Zilá Luz Paulino Simões
• Modalidade de apoio: Auxílio à Pesquisa - Regular