| Full text | |
| Author(s): |
de Carli, Gabriel Jose
[1, 2]
;
Rotela, Abdon Troche
[3, 4]
;
Lubini, Greice
[1]
;
Contiliani, Danyel Fernandes
[1, 2]
;
Candia, Nidia Benitez
[3]
;
Depintor, Thiago S.
[2]
;
Pinto de Abreu, Fabiano Carlos
[1, 2]
;
Paulino Simoes, Zila Luz
[1, 2]
;
Rios, Danilo Fernandez
[3]
;
Pereira, Tiago Campos
[1, 2]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[3] Univ Nacl Asuncion, Fac Ciencias Exactas & Nat, San Lorenzo - Paraguay
[4] Univ Nacl Asuncion, Fac Politecn, San Lorenzo - Paraguay
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 1 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
RNA interference (RNAi) is a powerful gene silencing technology, widely used in analyses of reverse genetics, development of therapeutic strategies and generation of biotechnological products. Here we present a free software tool for the rational design of RNAi effectors, named siRNA and shRNA designer (SSD). SSD incorporates our previously developed software Strand Analysis to construct template DNAs amenable for the large scale production of mono-, bi- and trivalent multimeric shRNAs, via in vitro rolling circle transcription. We tested SSD by creating a trivalent multimeric shRNA against the vitellogenin gene of Apis mellifera. RT-qPCR analysis revealed that our molecule promoted a decrease in more than 50% of the target mRNA, in a dose-dependent manner, when compared to the control group. Thus, SSD software allows the easy design of multimeric shRNAs, for single or multiple simultaneous knockdowns, which is especially interesting for studies involving large amounts of double-stranded molecules. (AU) | |
| FAPESP's process: | 16/06657-0 - Analysis of the endocrine system and circadian cycle as modulators of Apis mellifera development |
| Grantee: | Zilá Luz Paulino Simões |
| Support Opportunities: | Regular Research Grants |