How deep can ectomycorrhizas go? A case study on Pisolithus down to 4 meters in a Brazilian eucalypt plantation

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Robin, Agnes ^{[1, 2, 3]} ; Pradier, Celine ^{[2, 4]} ; Sanguin, Herve ^{[5, 6]} ; Mahe, Frederic ^{[5, 6]} ; Lambais, George Rodrigues ^[7] ; de Araujo Pereira, Arthur Prudencio ^{[1, 8]} ; Germon, Amandine ^[9] ; Santana, Maiele Cintra ^[1] ; Tisseyre, Pierre ^[10] ; Pablo, Anne-Laure ^[2] ; Heuillard, Pauline ^[11] ; Sauvadet, Marie ^[2] ; Bouillet, Jean-Pierre ^{[2, 3]} ; Andreote, Fernando Dini ^[1] ; Plassard, Claude ^[2] ; de Moraes Goncalves, Jose Leonardo ^[1] ; Bran Nogueira Cardoso, Elke Jurandy ^[1] ; Laclau, Jean-Paul ^{[2, 4]} ; Hinsinger, Philippe ^[2] ; Jourdan, Christophe ^{[2, 4]}

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^[1] Univ Sao Paulo, ESALQ, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Montpellier, Eco & Sols, CIRAD, INRA, IRD, Montpellier SupAgro, Montpellier - France

^[3] CIRAD, UMR Eco & Sols, BR-13418900 Piracicaba, SP - Brazil

^[4] CIRAD, UMR ECO & SOLS, F-34398 Montpellier - France

^[5] CIRAD, UMR BGPI, F-34398 Montpellier - France

^[6] Univ Montpellier, BGPI, CIRAD, INRA, IRD, Montpellier SupAgro, Montpellier - France

^[7] Univ Sao Paulo, CENA, BR-13418900 Piracicaba, SP - Brazil

^[8] Univ Fed Ceara, BR-60455760 Fortaleza, Ceara - Brazil

^[9] Univ Sao Paulo, UNESP, BR-18610300 Botucatu, SP - Brazil

^[10] Univ Montpellier, LSTM, CIRAD, INRA, IRD, Montpellier SupAgro, Montpellier - France

^[11] INRA, US 1426, GeT PlaGe, Genotoul, F-31320 Castanet Tolosan - France

• Tipo de documento: Artigo Científico
• Fonte: MYCORRHIZA; v. 29, n. 6, p. 637-648, NOV 2019.
• Citações Web of Science: 0

Resumo

Despite the strong ecological importance of ectomycorrhizal (ECM) fungi, their vertical distribution remains poorly understood. To our knowledge, ECM structures associated with trees have never been reported in depths below 2 meters. In this study, fine roots and ECM root tips were sampled down to 4-m depth during the digging of two independent pits differing by their water availability. A meta-barcoding approach based on Illumina sequencing of internal transcribed spacers (ITS1 and ITS2) was carried out on DNA extracted from root samples (fine roots and ECM root tips separately). ECM fungi dominated the rootassociated fungal community, with more than 90% of sequences assigned to the genus Pisolithus. The morphological and barcoding results demonstrated, for the first time, the presence of ECM symbiosis down to 4-m. The molecular diversity of Pisolithus spp. was strongly dependent on depth, with soil pH and soil water content as primary drivers of the Pisolithus spp. structure. Altogether, our results highlight the importance to consider the ECM symbiosis in deep soil layers to improve our understanding of fine roots functioning in tropical soils. (AU)

• Processo FAPESP: 16/18944-3 - Mudanças climáticas e eficiência energética na agricultura: um enfoque em estresse hídrico, manejo orgânico e biologia do solo
• Beneficiário: Elke Jurandy Bran Nogueira Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático