Isolation of Naegleria spp. from a Brazilian Water Source

Autor(es)

Bellini, Natalia Karla ^{[1, 2]} ; Moreira da Fonseca, Ana Leticia ^[1] ; Reyes-Batlle, Maria ^[2] ; Lorenzo-Morales, Jacob ^[2] ; Rocha, Odete ^[3] ; Thiemann, Otavio Henrique ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Fis Sao Carlos, Caixa Postal 369, BR-13560590 Sao Carlos, SP - Brazil

^[2] Univ La Laguna, Inst Univ Enfermedades Trop & Salud Publ Canarias, Avda Astrofis Fco Sanchez S-N, Tenerife 38203, Canary Islands - Spain

^[3] Univ Fed Sao Carlos, Dept Ecol & Biol Evolut, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13560590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS; v. 9, n. 2 FEB 2020.
• Citações Web of Science: 0

Resumo

The genus Naegleria, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. Naegleria fowleri, colloquially known as the ``brain-eating amoeba,{''} is the most studied Naegleria species because it causes primary amoebic meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation, we present research on identifying Naegleria spp. in the Monjolinho River as a model study. The river is a public Brazilian freshwater source that crosses the city of Sao Carlos, in Sao Paulo state, Brazil. Five distinct sampling sites were examined through limnological features, trophozoites culturing, and PCR against internal transcribed spacer (ITS) regions and 5.8S rRNA sequences. The results identified N. philippinensis, N. canariensisi, N. australiensis, N. gruberi, N. dobsoni sequences, as well as a Hartmannella sequence. The methodology delineated here represents the first Brazilian Naegleria spp. study on a freshwater system. Our results stress the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil. (AU)

• Processo FAPESP: 18/20693-4 - Diversidade de amebas de vida livre no Rio Monjolinho no estado de São Paulo - abordagens morfológicas e moleculares
• Beneficiário: Otavio Henrique Thiemann
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular