| Full text | |
| Author(s): |
Bellini, Natalia Karla
[1, 2]
;
Moreira da Fonseca, Ana Leticia
[1]
;
Reyes-Batlle, Maria
[2]
;
Lorenzo-Morales, Jacob
[2]
;
Rocha, Odete
[3]
;
Thiemann, Otavio Henrique
[1, 4]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Fis Sao Carlos, Caixa Postal 369, BR-13560590 Sao Carlos, SP - Brazil
[2] Univ La Laguna, Inst Univ Enfermedades Trop & Salud Publ Canarias, Avda Astrofis Fco Sanchez S-N, Tenerife 38203, Canary Islands - Spain
[3] Univ Fed Sao Carlos, Dept Ecol & Biol Evolut, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[4] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13560590 Sao Carlos, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PATHOGENS; v. 9, n. 2 FEB 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
The genus Naegleria, of the free-living amoeba (FLA) group, has been investigated mainly due to its human health impact, resulting in deadly infections and their worldwide distribution on freshwater systems. Naegleria fowleri, colloquially known as the ``brain-eating amoeba,{''} is the most studied Naegleria species because it causes primary amoebic meningoencephalitis (PAM) of high lethality. The assessment of FLA biodiversity is fundamental to evaluate the presence of pathogenic species and the possibility of human contamination. However, the knowledge of FLA distribution in Brazil is unknown, and to rectify this situation, we present research on identifying Naegleria spp. in the Monjolinho River as a model study. The river is a public Brazilian freshwater source that crosses the city of Sao Carlos, in Sao Paulo state, Brazil. Five distinct sampling sites were examined through limnological features, trophozoites culturing, and PCR against internal transcribed spacer (ITS) regions and 5.8S rRNA sequences. The results identified N. philippinensis, N. canariensisi, N. australiensis, N. gruberi, N. dobsoni sequences, as well as a Hartmannella sequence. The methodology delineated here represents the first Brazilian Naegleria spp. study on a freshwater system. Our results stress the urgency of a large scale evaluation of the presence of free-living amoebas in Brazil. (AU) | |
| FAPESP's process: | 18/20693-4 - Free living amoeba diversity on Monjolinho River at State of São Paulo - morphological and molecular approaches |
| Grantee: | Otavio Henrique Thiemann |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |