Bothrops moojeni venom and BmooLAAO-I downmodulate CXCL8/IL-8 and CCL2/MCP-1 production and oxidative burst response, and upregulate CD11b expression in human neutrophils

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Pereira-Crott, Luciana Simon ^[1] ; Casare-Ogasawara, Tania Mara ^[1] ; Ambrosio, Luciana ^[1] ; Princi Chaim, Luiz Fernando ^[1] ; de Morais, Fabiana Rosseto ^[1] ; Oliveira Cintra, Adelia Cristina ^[1] ; Canicoba, Nathalia Cristina ^[1] ; Fortunato Tucci, Luiz Fernando ^[1] ; Torqueti, Maria Regina ^[1] ; Sampaio, Suely Vilela ^[1] ; Marzocchi-Machado, Cleni Mara ^[1] ; de Castro, Fabiola Attie ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Immunopharmacology; v. 80, MAR 2020.
• Citações Web of Science: 1

Resumo

Bothrops snake venoms contain biologically active components, including L-amino acid oxidases (LAAO) that induce significant leukocyte accumulation at inflammatory sites characterized by early neutrophil infiltration. As it remains unclear how snake venoms modulate neutrophil activation and chemokine production, here we examined whether Bothrops moojeni crude venom (BmV) and its LAAO (BmooLAAO-I) affect expression of the surface activation markers CD11b and CD66b, production of the chemokines CCL2/MCP-1, CCLS/RANTES, CXCL8/IL-8, CXCL9/MIG, and CXCL-10/IP-10, and activation of oxidative burst in human neutrophils. Cell viability, expression of activation markers, and chemokine production were assessed by flow cytometry, while the oxidative burst response was measured by chemiluminescence. BmV at 50 and 75 mu g/mL reduced CXCL8/IL-8 (p < 0.001 and p < 0.01, respectively) and CCL2/MCP-1 production (p < 0.05), while BmooLAAO-I at the same concentrations reduced only CCL2/MCP-1 production (p < 0.01). These effects were accompanied by CD11b upregulation (p < 0.05 for 50 and 75 mu g/mL BmV; p < 0.01 for 50 and 75 mu g/mL BmooLAAO-I) and CD66b downregulation (p < 0.05 for 50 and 75 mu g/mL BmV). Both BmV and BmooLAAO-I at concentrations ranging from 0.625 to 5 mu g/mL suppressed the oxidative burst of neutrophils stimulated with phorbol 12-myristate 13-acetate, while BmooLAAO-I at 2.5 and 5 mu g/mL also suppressed the neutrophil response stimulated with opsonized zymosan. Considering that neutrophils participate in the pathogenesis of autoimmune and inflammatory diseases, the findings reported herein indicate that BmV and BmooLAAO-I are potential immunomodulating agents. (AU)

• Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
• Beneficiário: Suely Vilela
• Modalidade de apoio: Auxílio à Pesquisa - Temático