Does photobiomodulation change the synthesis and secretion of angiogenic proteins by different pulp cell lineages?

Autor(es):Mostrar menos -

Ribeiro Vitor, Luciana Lourenco ^[1] ; Oliveira Prado, Mariel Tavares ^[2] ; Neto, Natalino Lourenco ^[2] ; Oliveira, Rodrigo Cardoso ^[3] ; Sakai, Vivien Thiemy ^[4] ; Santos, Carlos Ferreira ^[3] ; Dionisio, Thiago Jose ^[3] ; Rios, Daniela ^[2] ; Cruvinel, Thiago ^[2] ; Andrade Moreira Machado, Maria Aparecida ^[2] ; Oliveira, Thais Marchini ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sagrado Coracao, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[4] Univ Fed Alfenas, Sch Dent, Dept Clin & Surg, Alfenas, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 203, JAN 2020.
• Citações Web of Science: 0

Resumo

This study aimed to compare the synthesis and secretion of VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, and FGF-2 between pulp fibroblasts from human primary teeth (HPF) and stem cell from human deciduous teeth (SHED) before and after photobiomodulation. HPF were obtained from explant technique and characterized by immunohistochemistry, while SHED were obtained from digestion technique and characterized by flow cytometry. HPF (control group) and SHED were plated, let to adhere, and put on serum starvation to synchronize the cell cycles prior to photobiomodulation. Then, both cell lineages were irradiated with 660-nm laser according to the following groups: 2.5 and 3.7 J/cm(2). MTT and crystal violet assays respectively verified viability and proliferation. ELISA Multiplex Assay assessed the following proteins: VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, FGF-2, at 6, 12, and 24 h after photobiomodulation, in supernatant and lysate. Two-way ANOVA/Tukey test evaluated cell viability and proliferation, while angiogenic production and secretion values were analyzed by one-way ANOVA (P < .05). Statistically similar HPF and SHED viability and proliferation patterns occurred before and after photobiomodulation (P > .05). HPF exhibited statistically greater values of all angiogenic proteins than did SHED, at all study periods, except for FGF-2 (supernatant; 12 h); VEGFR1 (lysate; non-irradiated; 12 h); and VEGFR1 (lysate; non-irradiated; 24 h). Photobiomodulation changed the synthesis and secretion of angiogenic proteins by HPF. HPF produced and secreted greater values of all tested angiogenic proteins than did SHED before and after irradiation with both energy densities of 2.5 and 3.7 J/cm(2). (AU)

• Processo FAPESP: 18/20316-6 - Análise da expressão gênica e síntese proteica de células derivadas da polpa de dentes decíduos após o uso do Laser de Baixa Intensidade.
• Beneficiário: Thais Marchini de Oliveira Valarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/18886-5 - Influência de diferentes densidades de energia do laser de baixa intensidade em células derivadas da polpa de dentes decíduos
• Beneficiário: Thais Marchini de Oliveira Valarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular