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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Does photobiomodulation change the synthesis and secretion of angiogenic proteins by different pulp cell lineages?

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Author(s):
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Ribeiro Vitor, Luciana Lourenco [1] ; Oliveira Prado, Mariel Tavares [2] ; Neto, Natalino Lourenco [2] ; Oliveira, Rodrigo Cardoso [3] ; Sakai, Vivien Thiemy [4] ; Santos, Carlos Ferreira [3] ; Dionisio, Thiago Jose [3] ; Rios, Daniela [2] ; Cruvinel, Thiago [2] ; Andrade Moreira Machado, Maria Aparecida [2] ; Oliveira, Thais Marchini [2]
Total Authors: 11
Affiliation:
[1] Univ Sagrado Coracao, Bauru, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP - Brazil
[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[4] Univ Fed Alfenas, Sch Dent, Dept Clin & Surg, Alfenas, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 203, JAN 2020.
Web of Science Citations: 0
Abstract

This study aimed to compare the synthesis and secretion of VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, and FGF-2 between pulp fibroblasts from human primary teeth (HPF) and stem cell from human deciduous teeth (SHED) before and after photobiomodulation. HPF were obtained from explant technique and characterized by immunohistochemistry, while SHED were obtained from digestion technique and characterized by flow cytometry. HPF (control group) and SHED were plated, let to adhere, and put on serum starvation to synchronize the cell cycles prior to photobiomodulation. Then, both cell lineages were irradiated with 660-nm laser according to the following groups: 2.5 and 3.7 J/cm(2). MTT and crystal violet assays respectively verified viability and proliferation. ELISA Multiplex Assay assessed the following proteins: VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, FGF-2, at 6, 12, and 24 h after photobiomodulation, in supernatant and lysate. Two-way ANOVA/Tukey test evaluated cell viability and proliferation, while angiogenic production and secretion values were analyzed by one-way ANOVA (P < .05). Statistically similar HPF and SHED viability and proliferation patterns occurred before and after photobiomodulation (P > .05). HPF exhibited statistically greater values of all angiogenic proteins than did SHED, at all study periods, except for FGF-2 (supernatant; 12 h); VEGFR1 (lysate; non-irradiated; 12 h); and VEGFR1 (lysate; non-irradiated; 24 h). Photobiomodulation changed the synthesis and secretion of angiogenic proteins by HPF. HPF produced and secreted greater values of all tested angiogenic proteins than did SHED before and after irradiation with both energy densities of 2.5 and 3.7 J/cm(2). (AU)

FAPESP's process: 18/20316-6 - Gene expression and protein synthesis analysis of dental pulp cells from deciduous teeth after the use of Low Intensity Laser.
Grantee:Thais Marchini de Oliveira Valarelli
Support Opportunities: Regular Research Grants
FAPESP's process: 13/18886-5 - Low intensity laser effects of different energy densities in cells of the pulp of human deciduous teeth
Grantee:Thais Marchini de Oliveira Valarelli
Support Opportunities: Regular Research Grants