Low intensity laser effects of different energy densities in cells of the pulp of human deciduous teeth

• Grant number: 13/18886-5
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2015
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Thais Marchini de Oliveira Valarelli
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The objective of this study is to investigate the Low Intensity Laser effects of different energy densities on growth and viability of the pulp of human deciduous teeth; expression of the VEGF, FGF-2, collagen I and DMP-1. Samples of pulp fibroblasts will be obtained and grown in specific culture of human deciduous teeth. After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: GI - Ia (1,2 J/cm2 - 05 mW - 10s), Ib (2,5 J/cm2 - 05 mW - 20s), Ic (3,7 J/cm2 - 05 mW - 30s), Id (5,0 J/cm2 - 05 mW - 40s), Ie (6,2 J/cm2 - 05 mW - 50s), If (Não irradiados); G II - IIa (1,2 J/cm2 - 05 mW - 10s), IIb (2,5 J/cm2 - 10 mW - 10s), IIc (3,7 J/cm2 - 15 mW - 10s), IId (5,0 J/cm2 - 20 mW - 10s), IIe (6,2 J/cm2 - 25 mW - 10s), IIf (no irradiation). After irradiation the cells will be collected for the experiments of cell viability and growth. The techniques used for the analysis will be: MTT, RT-PCR and ELISA. The data will be described by means of graphs and tables, by using ANOVA test followed by Tukey test. If necessary, other tests may be applied. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA PRADO, MARIE TAVARES; RIBEIRO VITOR, LUCIANA LOURENGO; LOURENCO NETO, NATALINO; TEIXEIRA MARQUES, NADIA CAROLINA; SAKAI, VIVIEN THIEMY; RIOS, DANIELA; CRUVINEL, THIAGO; OLIVEIRA, RODRIGO CARDOSO; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; et al. Effects of different culture media, cell densities and adhesion periods on stem cells from human exfoliated deciduous teeth after photobiomodulation. LASER PHYSICS LETTERS, v. 16, n. 9, SEP 2019. (15/19696-0, 13/18886-5)

RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA PRADO, MARIEL TAVARES; LOURENCO NETO, NATALINO; DE OLIVEIRA, RODRIGO CARDOSO; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Photobiomodulation changes type 1 collagen gene expression by pulp fibroblasts. Laser Physics, v. 28, n. 6, JUN 2018. (13/18886-5)

TEIXEIRA MARQUES, NADIA CAROLINA; LOURENCO NETO, NATALINO; OLIVEIRA PRADO, MARIEL TAVARES; RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA, RODRIGO CARDOSO; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Effects of PBM in different energy densities and irradiance on maintaining cell viability and proliferation of pulp fibroblasts from human primary teeth. Lasers in Medical Science, v. 32, n. 7, p. 1621-1628, SEP 2017. (13/16156-0, 13/18886-5, 15/19696-0)

RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA PRADO, MARIEL TAVARES; NETO, NATALINO LOURENCO; OLIVEIRA, RODRIGO CARDOSO; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; DIONISIO, THIAGO JOSE; RIOS, DANIELA; CRUVINEL, THIAGO; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; et al. Does photobiomodulation change the synthesis and secretion of angiogenic proteins by different pulp cell lineages?. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 203, JAN 2020. (18/20316-6, 13/18886-5)

FERNANDES, AMANDA; LOURENCO NETO, NATALINO; TEIXEIRA MARQUES, NADIA CAROLINA; RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA PRADO, MARIEL TAVARES; OLIVEIRA, RODRIGO CARDOSO; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Cellular response of pulp fibroblast to single or multiple photobiomodulation applications. Laser Physics, v. 28, n. 6, JUN 2018. (13/18886-5)