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Protein gene expression by stem cells from pulp of deciduous teeth after the use of low intensity laser

Grant number: 17/11396-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2018
Effective date (End): March 31, 2020
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal researcher:Thais Marchini de Oliveira Valarelli
Grantee:Mariel Tavares de Oliveira Prado Bergamo
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):18/13675-0 - Dental pulp stem cell biology., BE.EP.DR

Abstract

This study aimed to evaluate the effect of different energy densities of Low Intensity Laser on cell viability and proliferation of stem cell from human exfoliated deciduous teeth and dental pulp cells; on the RNAm expression of FGF2, VEGF, DSPP and DMP-1; and on the protein expression of FGF2, VEGF, DSPP and DMP-1. Cells samples will be obtained and grown in MEM± e DMEM culture medium with Fetal Bovine Serum (FBS). After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: Group 1(1.2 J/cm2 - 05 mW - 10s), Group 2(2.5 J / cm2 - 05 mW - 20s), Group 3(3.7 J/cm2 - 05 mW - 30s), Group 4(5.0 J/cm2 - 05 mW - 40s), Group 5(6.2 J/cm2 - 05 mW - 50s), Group 6(2.5 J/cm2 - 10 mW - 10s), Group 7(3.7 J/cm2 - 15 mW - 10s), Group 8( 5.0 J/cm2 - 20 mW - 10 sec), Group 9 (6.2 J/cm2 - 25 mW - 10 sec), Positive Control (MEM± +10% FBS - no irradiated), Negative Control (MEM± +1% FBS - no irradiated). The techniques used for the analysis will be MTT, Crystal Violet, RT-PCR, ELISA, Western Blot and Immunofluorescence. The values in triplicate will be analyzed by ANOVA followed by Tukey test and Kruskal-Wallis analysis complemented by Dunn's test when obey normal and non-normal distribution, respectively. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BERGAMO, MARIEL TAVARES; VITOR, LUCIANA LOURENCO RIBEIRO; DIONISIO, THIAGO JOSE; MARQUES, NADIA CAROLINA TEIXEIRA; OLIVEIRA, RODRIGO CARDOSO; AMBROSIO, ELOA CRISTINA PASSUCCI; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; LOURENCO NETO, NATALINO; MACHADO, MARIA APARECIDA ANDRADE MOREIRA; OLIVEIRA, THAIS MARCHINI. Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?. Lasers in Medical Science, v. 36, n. 8, p. 1751-1758, OCT 2021. Web of Science Citations: 1.
BERGAMO, MARIEL TAVARES; VITOR, LUCIANA LOURENCO RIBEIRO; DIONISIO, THIAGO JOSE; MARQUES, NADIA CAROLINA TEIXEIRA; OLIVEIRA, RODRIGO CARDOSO; AMBROSIO, ELOA CRISTINA PASSUCCI; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; LOURENCO NETO, NATALINO; MACHADO, MARIA APARECIDA ANDRADE MOREIRA; OLIVEIRA, THAIS MARCHINI. Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?. Lasers in Medical Science, APR 2021. Web of Science Citations: 0.
OLIVEIRA PRADO BERGAMO, MARIEL TAVARES; RIBEIRO VITOR, LUCIANA LOURENCO; LOPES, NATHALIA MARTINS; LOURENCO NETO, NATALINO; DIONISIO, THIAGO JOSE; OLIVEIRA, RODRIGO CARDOSO; RODINI, CAMILA OLIVEIRA; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study. Lasers in Medical Science, v. 35, n. 9 FEB 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.