Advanced search
Start date
Betweenand

Low intensity laser effects of different energy densities in fibroblasts of the pulp of human deciduous teeth

Grant number: 13/16156-0
Support Opportunities:Scholarships in Brazil - Doctorate
Start date: December 01, 2014
End date: December 31, 2015
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Thais Marchini de Oliveira Valarelli
Grantee:Nádia Carolina Teixeira Marques
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The objective of this study is to investigate the Low Intensity Laser effects of different energy densities on growth and viability of the fibroblasts of human deciduous teeth; expression of the VEGF, FGF-2, collagen I and DMP-1. Samples of pulp fibroblasts will be obtained and grown in specific culture of human deciduous teeth. After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: GI - Ia (1,2 J/cm2 - 05 mW - 10s), Ib (2,5 J/cm2 - 05 mW - 20s), Ic (3,7 J/cm2 - 05 mW - 30s), Id (5,0 J/cm2 - 05 mW - 40s), Ie (6,2 J/cm2 - 05 mW - 50s), If (Não irradiados); G II - IIa (1,2 J/cm2 - 05 mW - 10s), IIb (2,5 J/cm2 - 10 mW - 10s), IIc (3,7 J/cm2 - 15 mW - 10s), IId (5,0 J/cm2 - 20 mW - 10s), IIe (6,2 J/cm2 - 25 mW - 10s), IIf (no irradiation). After irradiation the cells will be collected for the experiments of cell viability and growth. The techniques used for the analysis will be: MTT, RT-PCR, Elisa and Immunofluorescent. The results will be submitted to appropriate statistical analysis. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TEIXEIRA MARQUES, NADIA CAROLINA; LOURENCO NETO, NATALINO; OLIVEIRA PRADO, MARIEL TAVARES; RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA, RODRIGO CARDOSO; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Effects of PBM in different energy densities and irradiance on maintaining cell viability and proliferation of pulp fibroblasts from human primary teeth. Lasers in Medical Science, v. 32, n. 7, p. 1621-1628, . (13/16156-0, 13/18886-5, 15/19696-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARQUES, Nádia Carolina Teixeira. Influence of low-level laser with different energy densities on pulp fibroblasts from human primary teeth. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.