Low intensity laser effects of different energy densities in fibroblasts of the pulp of human deciduous teeth

• Grant number: 13/16156-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2014
• End date: December 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Nádia Carolina Teixeira Marques
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The objective of this study is to investigate the Low Intensity Laser effects of different energy densities on growth and viability of the fibroblasts of human deciduous teeth; expression of the VEGF, FGF-2, collagen I and DMP-1. Samples of pulp fibroblasts will be obtained and grown in specific culture of human deciduous teeth. After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: GI - Ia (1,2 J/cm2 - 05 mW - 10s), Ib (2,5 J/cm2 - 05 mW - 20s), Ic (3,7 J/cm2 - 05 mW - 30s), Id (5,0 J/cm2 - 05 mW - 40s), Ie (6,2 J/cm2 - 05 mW - 50s), If (Não irradiados); G II - IIa (1,2 J/cm2 - 05 mW - 10s), IIb (2,5 J/cm2 - 10 mW - 10s), IIc (3,7 J/cm2 - 15 mW - 10s), IId (5,0 J/cm2 - 20 mW - 10s), IIe (6,2 J/cm2 - 25 mW - 10s), IIf (no irradiation). After irradiation the cells will be collected for the experiments of cell viability and growth. The techniques used for the analysis will be: MTT, RT-PCR, Elisa and Immunofluorescent. The results will be submitted to appropriate statistical analysis. The significance level of 5% will be adopted for the differences considered statistically significant. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TEIXEIRA MARQUES, NADIA CAROLINA; LOURENCO NETO, NATALINO; OLIVEIRA PRADO, MARIEL TAVARES; RIBEIRO VITOR, LUCIANA LOURENCO; OLIVEIRA, RODRIGO CARDOSO; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Effects of PBM in different energy densities and irradiance on maintaining cell viability and proliferation of pulp fibroblasts from human primary teeth. Lasers in Medical Science, v. 32, n. 7, p. 1621-1628, SEP 2017. (13/16156-0, 13/18886-5, 15/19696-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARQUES, Nádia Carolina Teixeira. Influence of low-level laser with different energy densities on pulp fibroblasts from human primary teeth. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.