Gene and immunohistochemical expression of HIF-1 alpha, GLUT-1, FASN, and adipophilin in carcinoma ex pleomorphic adenoma development

Autor(es):Mostrar menos -

Scarini, Joao Figueira ^[1] ; Rosa, Larissa Fernandes ^[2] ; de Lima Souza, Reydson Alcides ^[1] ; Abu Egal, Erika Said ^[2] ; Tincani, Alfio Jose ^[3] ; Martins, Antonio Santos ^[3] ; Kowalski, Luiz Paulo ^[4] ; Graner, Edgard ^[1] ; Della Coletta, Ricardo ^[1] ; Carlos, Roman ^[5] ; Gondak, Rogerio de Oliveira ^[6] ; de Almeida, Oslei Paes ^[1] ; de Almeida Milani Altemani, Albina Messias ^[2] ; Bastos, Debora Campanella ^[1] ; Mariano, Fernanda Viviane ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Oral Diag Dept, Piracicaba Dent Sch, Piracicaba - Brazil

^[2] Univ Estadual Campinas, Pathol Dept, Fac Med Sci, Campinas - Brazil

^[3] Univ Estadual Campinas, Head & Neck Surg Dept, Fac Med Sci, Campinas - Brazil

^[4] AC Camargo Canc Ctr, Head & Neck Surg Dept, Sao Paulo - Brazil

^[5] Head & Neck Clin Ctr, Oral & Maxillofacial Pathol Lab, Guatemala City - Guatemala

^[6] Fed Univ Santa Catarina UFSC, Dept Pathol, Florianopolis, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 26, n. 6 APR 2020.
• Citações Web of Science: 0

Resumo

Objective To analyze the gene and immunohistochemical expression of HIF-1 alpha, GLUT-1, FASN, and adipophilin in normal salivary gland (NSG), pleomorphic adenoma (PA), and carcinoma ex pleomorphic adenoma (CXPA) samples. Material and Methods The gene expression was investigated by the real-time PCR (qRT-PCR) method in 9 samples of frozen tissues of normal salivary gland, 13 PA, and 10 CXPA. We validated the reactions by immunohistochemistry on 20 samples from NSG, 85 PA, and 44 CXPA. Results Our results showed that there was no statistically significant difference in HIF-1 alpha gene and immunohistochemistry expression among the tissues studied while FASN gene and immunohistochemistry expression increased along the carcinogenesis of the PA. GLUT-1 was significantly more expressed in tumor tissues (PA and CXPA), although protein is mainly expressed in transformed cells than in PA and NSG. In contrast, adipophilin was significantly more expressed in NSG while the expression of the protein increased in PA and CXPA. Conclusions In summary, the data presented here suggest that neoplastic cells reprogram the expression of GLUT-1 and adipophilin to adapt to the tumor microenvironment and reinforce, through immunohistochemical results, a possible transcriptional and post-translational regulatory mechanisms that act on the expression of these genes. (AU)

• Processo FAPESP: 15/07304-0 - Estudo das alterações genéticas e metabólicas do adenoma pleomorfo e carcinoma ex-adenoma pleomorfo por exoma, expressão gênica e imunohistoquímica
• Beneficiário: Fernanda Viviane Mariano Brum Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/00831-0 - Estudo das alterações genéticase metabólicas do adenoma pleomorfo e carcinoma ex-adenoma pleomorfo por exoma, expressão gênica e imunohistoquímica
• Beneficiário: Fernanda Viviane Mariano Brum Corrêa
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 17/13167-1 - Estudo das alterações metabólicas do adenoma pleomorfo e carcinoma ex-adenoma por imuno-histoquímica
• Beneficiário: Larissa Fernandes Rosa
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica