Experimental models and methods for cutaneous wound healing assessment

Autor(es)

Masson-Meyers, Daniela S. ^{[1, 2]} ; Andrade, Thiago A. M. ^{[1, 3]} ; Caetano, Guilherme F. ^{[1, 3]} ; Guimaraes, Francielle R. ^{[1, 4]} ; Leite, Marcel N. ^[1] ; Leite, Saulo N. ^{[1, 5]} ; Frade, Marco Andrey C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Div Dermatol, Ribeirao Preto, SP - Brazil

^[2] Marquette Univ, Sch Dent, 1801 West Wisconsin Ave, Milwaukee, WI 53233 - USA

^[3] Univ Ctr Herminio Ometto Fdn FHO, Grad Program Biomed Sci, Araras, SP - Brazil

^[4] Univ Ctr Associated Sch Educ UNIFAE, Sao Joao Da Boa Vista, SP - Brazil

^[5] Univ Ctr Educ Fdn Guaxupe UNIFEG, Guaxupe, MG - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: International Journal of Experimental Pathology; v. 101, n. 1-2 MAR 2020.
• Citações Web of Science: 0

Resumo

Wound healing studies are intricate, mainly because of the multifaceted nature of the wound environment and the complexity of the healing process, which integrates a variety of cells and repair phases, including inflammation, proliferation, reepithelialization and remodelling. There are a variety of possible preclinical models, such as in mice, rabbits and pigs, which can be used to mimic acute or impaired for example, diabetic and nutrition-related wounds. These can be induced by many different techniques, with excision or incision being the most common. After determining a suitable model for a study, investigators need to select appropriate and reproducible methods that will allow the monitoring of the wound progression over time. The assessment can be performed by non-invasive protocols such as wound tracing, photographic documentation (including image analysis), biophysical techniques and/or by invasive protocols that will require wound biopsies. In this article, we provide an overview of some of the most often needed and used: (a) preclinical/animal models including incisional, excisional, burn and impaired wounds; (b) methods to evaluate the healing progression such as wound healing rate, wound analysis by image, biophysical assessment, histopathological, immunological and biochemical assays. The aim is to help researchers during the design and execution of their wound healing studies. (AU)

• Processo FAPESP: 14/23662-1 - Produção de scaffolds 3D contendo diferentes concentrações de grafeno, hidroxiapatita e fosfato tricálcico revestidos com proteína humana morfogenética óssea e P-1 do látex
• Beneficiário: Guilherme Ferreira Caetano
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/16437-7 - Avaliação da viabilidade do modelo de pele humana ex vivo (hOSEC) e sua aplicação em estudos de eficácia de protetores solares a longo prazo no fotoenvelhecimento
• Beneficiário: Marco Andrey Cipriani Frade
• Modalidade de apoio: Auxílio à Pesquisa - Regular