Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Autor(es)

Maruyama, Cintia Rodrigues ^{[1, 2]} ; Bilesky-Jose, Natalia ^[2] ; de Lima, Renata ^[2] ; Fraceto, Leonardo Fernandes ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Inst Sci & Technol Sorocaba, Environm Nanotechnol Lab, Sorocaba - Brazil

^[2] Univ Sorocaba, Lab Bioact Assessment & Toxicol Nanomat, Sorocaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 8, MAR 25 2020.
• Citações Web of Science: 0

Resumo

Trichoderma harzianum is a biological control agent used against phytopathogens and biostimulation in agriculture. However, its efficacy can be affected by biotic and abiotic factors, and microencapsulation has been used to maximize the efficacy. The objective was to develop polymeric microparticles to encapsulate T. harzianum, to perform physicochemical characterization to evaluate its stability, to evaluate effects on the soil microbiota, antifungal activity in vitro and enzymatic activity. Size distribution of wet and dry microparticles was 2000 and 800 mu m, respectively. Scanning electron microscopy showed spherical morphology and encapsulation of T. harzianum. Photostability assays showed that encapsulation protected the fungus against ultraviolet radiation. The evaluation of the microbiota showed that the proportion of denitrifying bacteria increased when compared to the control. The T. harzianum encapsulation showed an improvement in the chitinolytic and cellulosic activity. In vitro tests showed that encapsulated fungus were able to provide a greater control of S. sclerotiorum. (AU)

• Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
• Beneficiário: Leonardo Fernandes Fraceto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/18231-4 - Micropartículas poliméricas como sistema carreador do fungo Trichoderma harzianum visando aplicações em agricultura
• Beneficiário: Cintia Rodrigues Maruyama
• Modalidade de apoio: Bolsas no Brasil - Doutorado