"transcriptional analysis of Trichoderma harzianum CBMAI-0179 during cellulose and...
| Full text | |
| Author(s): |
Maruyama, Cintia Rodrigues
[1, 2]
;
Bilesky-Jose, Natalia
[2]
;
de Lima, Renata
[2]
;
Fraceto, Leonardo Fernandes
[1]
Total Authors: 4
|
| Affiliation: | [1] Sao Paulo State Univ, UNESP, Inst Sci & Technol Sorocaba, Environm Nanotechnol Lab, Sorocaba - Brazil
[2] Univ Sorocaba, Lab Bioact Assessment & Toxicol Nanomat, Sorocaba - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 8, MAR 25 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Trichoderma harzianum is a biological control agent used against phytopathogens and biostimulation in agriculture. However, its efficacy can be affected by biotic and abiotic factors, and microencapsulation has been used to maximize the efficacy. The objective was to develop polymeric microparticles to encapsulate T. harzianum, to perform physicochemical characterization to evaluate its stability, to evaluate effects on the soil microbiota, antifungal activity in vitro and enzymatic activity. Size distribution of wet and dry microparticles was 2000 and 800 mu m, respectively. Scanning electron microscopy showed spherical morphology and encapsulation of T. harzianum. Photostability assays showed that encapsulation protected the fungus against ultraviolet radiation. The evaluation of the microbiota showed that the proportion of denitrifying bacteria increased when compared to the control. The T. harzianum encapsulation showed an improvement in the chitinolytic and cellulosic activity. In vitro tests showed that encapsulated fungus were able to provide a greater control of S. sclerotiorum. (AU) | |
| FAPESP's process: | 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity |
| Grantee: | Leonardo Fernandes Fraceto |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/18231-4 - Polymeric microparticles as the fungus Trichoderma harzianum carrier system aimed at applications in agriculture |
| Grantee: | Cintia Rodrigues Maruyama |
| Support type: | Scholarships in Brazil - Doctorate |