Biomarkers of the oxidative stress and neurotoxicity in tissues of the bullfrog, Lithobates catesbeianus to assess exposure to metals

Autor(es)

Carvalho, Cleoni dos Santos ^{[1, 2]} ; Moraes Utsunomiya, Heidi Samantha ^{[1, 2]} ; Pasquoto-Stigliani, Tatiane ^[3] ; Costa, Monica Jones ^{[1, 2]} ; Fernandes, Marisa Narciso ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Programa Posgrad Biotecnol & Monitoramento Ambien, Campus Sorocaba, Rodovia Joao Leme dos Santos, BR-18052780 Sorocaba, SP - Brazil

^[3] Univ Sorocaba, Programa Posgrad Ciencias Farmaceut, Rodovia Raposo Tavares, Km 92, 5 Artura, BR-18023000 Sorocaba, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Ciencias Fisiol, Caixa Postal 676, Rodovia Washington Luis Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 196, JUN 15 2020.
• Citações Web of Science: 1

Resumo

Lithobates catesbeianus tadpoles were exposed to 1 mu g L-1 of zinc (Zn), copper (Cu) and cadmium (Cd) alone or combined (1:1 and 1:1:1) for 2 and 16 days. Results showed a significant increase in the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities in the liver, kidney and muscle (except for GPx) in the groups exposed to metal either alone or co-exposed after 2 days compared to the control. After 16 days, SOD, CAT and GST activities decreased significantly in the liver and kidney and GPx activity increased in the liver. Reduced glutathione (GSH) increased in the liver and kidney following combined exposure and decreased after 2 days of metal exposure in the muscle. There were significant increases in lipid hydroperoxide (LPO) levels in the liver, kidney and muscle (2 and 16 days), with the highest levels after metal co-exposure. Cholinesterase (ChE) activity increased significantly in the brain after 2 days of exposure but decreased in the brain (16 days) and muscle (2 days) after exposure to metals, alone and combined. The current study highlighted that the antioxidant system of L. catesbeianus was sensitive to metals and specially to the co-exposure of the three metals, despite presenting differences in the response among tissues. In addition, tadpoles were sensitive at both periods of exposure, but in different modes with stress response (activation, upregulation) at 2 days and oppression (down-regulation) at 16 days. (AU)

• Processo FAPESP: 17/23781-9 - Avaliação ecotoxicológica da água do rio Sorocaba utilizando uma análise integrada de biomarcadores bioquímicos e morfológicos em girinos de rã-touro, Lithobates catesbeianus (Shaw, 1802).
• Beneficiário: cleoni dos santos carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50752-3 - Biomarcadores de exposicao a metais em girinos de ra-touro.
• Beneficiário: cleoni dos santos carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular