Dps protein is related to resistance of Mycobacterium abscessus subsp. massiliense against stressful conditions

Autor(es)

de Alcantara, Nayra Rodrigues ^[1] ; de Oliveira, Fabio Muniz ^[1] ; Garcia, Wanius ^[2] ; Leitao dos Santos, Otavio Augusto ^[2] ; Junqueira-Kipnis, Ana Paula ^[1] ; Kipnis, Andre ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Goias, Inst Patol Trop & Saude Publ, Goiania, Go - Brazil

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Microbiology and Biotechnology; v. 104, n. 11 APR 2020.
• Citações Web of Science: 0

Resumo

Mycobacterium abscessus subsp. massiliense (Mycma) belongs to the Mycobacterium abscessus complex and is a rapidly growing non-tuberculous mycobacterium. The chronic pulmonary, skin, and soft tissue infections that it causes may be difficult to treat due to its intrinsic resistance to the commonly used antimicrobial drugs, making it a serious world public health problem. Iron is an essential nutrient for the growth of microorganisms; nonetheless, it can be toxic when in excess. Thus, bacteria require an iron homeostasis mechanism to succeed in different environments. DNA-binding proteins from starved cells (Dps) are miniferritins with the property to act as additional iron storage proteins but also can bind to DNA, protecting it against hydroxyl radical. Annotation of the Mycma genome revealed the gene mycma\_03135 with 79% sequential identity when compared to MSMEG\_3242 gene from M. smegmatis mc(2) 155, which codifies for a known Dps. Recombinant Dps from M. abscessus (rMaDps) was produced in Escherichia coli, purified in soluble form and shown to form high mass oligomers in solution with ferroxidase activity, DNA binding, and protection against damage. The expression of the mycma\_03135 gene was induced during Mycma growth in the presence of hydrogen peroxide (H2O2). Additionally, the expression of rMaDps by E. coli conferred greater resistance to H2O2. Thus, this study is the first to identify and characterize a Dps from M. abscessus. (AU)

• Processo FAPESP: 17/17275-3 - Estudos do modo de ação de duas mono-oxigenases líticas de polissacarídeos de inseto (ordem isoptera): estrutura molecular, química bioinorgânica e aplicações biotecnológicas
• Beneficiário: Wanius José Garcia da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular