| Texto completo | |
| Autor(es): |
Imamura, Amanda Hikari
[1, 2]
;
Segato, Thiago Pinotti
[1, 2]
;
Marques de Oliveira, Leticia Jordao
[1, 2]
;
Hassan, Ayaz
[1]
;
Crespilho, Frank Nelson
[1]
;
Carrilho, Emanuel
[1, 2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Quim Sao Carlos, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
[2] Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Microchimica Acta; v. 187, n. 5 APR 15 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
The oxidation of paper by periodate was investigated and systematically characterized by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy, X-ray diffraction, goniometry, and dynamic mechanical analysis. For the first time, in situ FTIR microscopy analysis was performed, yielding chemical images of carbonyl groups on the cellulose fibers. The enhancement of protein immobilization on oxidized paper was quantified by a colorimetric assay with Ponceau dye, demonstrating that 0.5-h oxidation suffices to functionalize the paper-based devices. The oxidized paper was applied as a sensor for protein quantification in urine, a test able to detect levels of proteinuria and even microalbuminuria. The quantification was based on the capture of proteins through covalent bonds formed with the carbonyl groups on the oxidized paper followed by the staining of the region with Ponceau dye. There is a linear dependency between human serum albumin (HSA) concentration and the length of the stained blot from 0.1 to 3 mg mL(-1). This method correlated linearly with a reference method showing a higher sensitivity (0.866 cm mL mg(-1)) than the latter. The limit of quantification was 0.1 mg mL(-1), three times lower than that of the commercial strip. (AU) | |
| Processo FAPESP: | 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica |
| Beneficiário: | Marco Aurelio Zezzi Arruda |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/25806-6 - Interação entre biomoléculas e nanoestruturas: eletroquímica, interfaces e superfícies |
| Beneficiário: | Ayaz Hassan |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico |
| Beneficiário: | Osvaldo Novais de Oliveira Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/05362-9 - Desenvolvimento de um biossensor wearable para determinação de cortisol em suor em dispositivo microfluídico à base de papel (uPAD) |
| Beneficiário: | Amanda Hikari Imamura |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |