Monitoring cellulose oxidation for protein immobilization in paper-based low-cost biosensors

Author(s)

Imamura, Amanda Hikari ^{[1, 2]} ; Segato, Thiago Pinotti ^{[1, 2]} ; Marques de Oliveira, Leticia Jordao ^{[1, 2]} ; Hassan, Ayaz ^[1] ; Crespilho, Frank Nelson ^[1] ; Carrilho, Emanuel ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Microchimica Acta; v. 187, n. 5 APR 15 2020.
• Web of Science Citations: 0

Abstract

The oxidation of paper by periodate was investigated and systematically characterized by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy, X-ray diffraction, goniometry, and dynamic mechanical analysis. For the first time, in situ FTIR microscopy analysis was performed, yielding chemical images of carbonyl groups on the cellulose fibers. The enhancement of protein immobilization on oxidized paper was quantified by a colorimetric assay with Ponceau dye, demonstrating that 0.5-h oxidation suffices to functionalize the paper-based devices. The oxidized paper was applied as a sensor for protein quantification in urine, a test able to detect levels of proteinuria and even microalbuminuria. The quantification was based on the capture of proteins through covalent bonds formed with the carbonyl groups on the oxidized paper followed by the staining of the region with Ponceau dye. There is a linear dependency between human serum albumin (HSA) concentration and the length of the stained blot from 0.1 to 3 mg mL(-1). This method correlated linearly with a reference method showing a higher sensitivity (0.866 cm mL mg(-1)) than the latter. The limit of quantification was 0.1 mg mL(-1), three times lower than that of the commercial strip. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/25806-6 - Interaction between biomolecules and nanostructures: electrochemistry, interfaces and surfaces
• Grantee: Ayaz Hassan
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/05362-9 - Development of wearable biosensor based on microfluidic paper-based analytical devices (uPAD) for cortisol analysis in sweat
• Grantee: Amanda Hikari Imamura
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)