Abstract
This Project will bring together four research groups from distinct institutions in order to investigate biologically-relevant nanostructured films, focusing on three topics: i) use of Langmuir monolayers, Langmuir-Blodgett (LB) films and vesicles as cell membrane models; ii) biosensors; and iii) single molecule detection (SMD). The films will be fabricated with the LB and layer-by-layer (LbL) techniques, from a variety of materials, and characterized with electrical measurements, microscopies and optical spectroscopies (already available), in addition to confocal microscopy, interface rheometry and ellipsometry with equipment to be acquired. With the expansion in infrastructure and upon integrating efforts from the four Groups, we hope to address scientific problems crucial for nanobiotechnology. Spectroscopic ellipsometry, for instance, will be useful to obtain parameters such as dielectric complex constant, absorption coefficient and thickness of the various types of films. With the FTIR microscopy coupled to electrochemistry, the mechanisms of interaction between biomolecules and solid surfaces can be elucidated in situ, which is useful for the design of new biodevices in clinical diagnosis. In cell membrane models, the purpose is to correlate properties of the molecules of interest with their physiological function. For the biosensors, a generic platform based on nanostructured films will be used to detect antigens or antibodies aimed at clinical diagnosis of neglected diseases and cancer. The data generated will be treated with information visualization and pattern recognition methods. In SMD, use will be made of surface-enhanced Raman scattering (SERS) to detect biologically-relevant molecules. In all the problems to be addressed, synergy will be sought with the expertise of the distinct research Groups. (AU)
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