| Full text | |
| Author(s): |
Wilson, Deivy
[1, 2]
;
Materon, Elsa M.
[2, 3]
;
Ibanez-Redin, Gisela
[2]
;
Faria, Ronaldo C.
[3]
;
Correa, Daniel S.
[1]
;
Oliveira, Jr., Osvaldo N.
[2]
Total Authors: 6
|
| Affiliation: | [1] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Chem, Rod Washington Luiz, Km 235, BR-13565970 Sao Carlos, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Talanta; v. 194, p. 611-618, MAR 1 2019. |
| Web of Science Citations: | 2 |
| Abstract | |
Outbreaks of foodbome diseases demand simple, rapid techniques for detecting pathogenic bacteria beyond the standard methods that are not applicable to routine analysis in the food industry and in the points of food consumption. In this work, we developed a sensitive, rapid and low-cost assay for detecting Escherichia colt (E.colt), Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. ryphi) in potable water and apple juice. The assay is based on electrical impedance spectroscopy measurements with screen-printed interdigitated electrodes coupled with magnetite nanoparticles functionalized with the antimicrobial peptide melittin (MLT). The data were analyzed with the information visualization methods Sammon's Mapping and Interactive Document Map to distinguish samples at two levels of contamination from food suitable for consumption. With this approach it has been possible to detect E. colt concentration down to 1 CFU mL(-1) in potable water and 3.5 CFU mL(-1) in apple juice without sample preparation, within only 25 min. This approach may serve as a low-cost, quick screening procedure to detect bacteria-related food poisoning, especially if the impedance data of several sensing units are combined. (AU) | |
| FAPESP's process: | 13/14262-7 - Nanostructured films from biologically-relevant materials |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/12174-4 - Development of hybrid polymer nanofibers for agricultural applications |
| Grantee: | Daniel Souza Corrêa |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 15/01770-0 - Development of analytical tools based on electronic tongues for simultaneous detection of bacterial threats |
| Grantee: | Deivy Wilson Masso |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |