The study and development of biosensors has focused on the use of microfluidic devices, functionalized with biomolecules, which allows miniaturization, low cost and quick results. In this work, biosensors will be developed from microfluidic devices functionalized in different molecular architectures for mastitis detection in milk samples. The first part of the project provides for the molecular architectures fabrication using self-assembly techniques, combining the properties of chitosan, chondroitin sulfate and sericin, and the characterization of these nanostructures. From the optimization of manufacturing conditions, the better architectures will be functionalized Anti-S-aureus antibodies to detect Staphylococcus aureus. Electrical impedance spectroscopy will be used to obtain calibration curves, which will be used in the establishment of adsorption models in biosensors and analyzes of milk samples contaminated with bacterias, obtained from Embrapa Gado de Leite. In addition to detection, selectivity and false positive biosensors analysis will be held through information visualization maps, allowing the optimization of the best conditions and parameters for specific sensing units. Finally, morphology and chemical interactions studies are performed through PM-IRRAS spectroscopy, and AFM analysis. The combination of characterization and detection techniques will optimize sensitivity and selectivity performance for mastitis detection and analysis of milk quality.
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