Development of nanostructured biosensors for the detection of endocrine disruptors in aquaculture

• Grant number: 13/26712-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2014
• End date: May 01, 2017
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Daniel Souza Corrêa
• Grantee: Adriana Pavinatto da Costa
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated scholarship(s): 15/08033-0 - Development os wearable electrochemical sensors and biosensors, BE.EP.PD

Abstract

This Project aims at the development of biosensors for detection of endocrine disruptor 17-a-ethinylestradiol in aquaculture. The 17-a-ethinylestradiol is a synthetic estrogen hormone and major constituent of birth control pills sold in Brazil. Its uncontrolled release in environment is often associated with changes in the endocrine system of fish and aquatic animals which have enduring contact with the hormone. The purpose is to develop a system that can detect this hormone with precision and at low cost. The sensor units will consist of enzyme or antibody immobilized on polyelectrolyte films or modified interdigitated electrodes coated with gold, using self-assembly layer-by-layer (LbL) and SAM techniques, respectively. The nanostructured films will be fabricated with different architectures in order to match synergy between the components of the film and the analytes. The nanostructured films growth will be monitored with various experimental techniques, including UV-Vis absorption and fluorescence spectroscopies, FTIR spectroscopy and atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) which will also serve to characterize the film properties. The detection will be carried out by using electrochemical techniques, e.g., cyclic voltammetry, squarewave voltammetry and amperometry in standard solutions and, subsequently, in real samples. We also intend to employ the concept of global selectivity of the electronic tongue along with the concept of biosensors in real water samples. As a large amount of data is expected from sensor data collection, information visualization methods will be used in the analysis, in order to optimize the sensitivity and selectivity of biosensors.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MERCANTE, LUIZA A.; SCAGION, VANESSA P.; PAVINATTO, ADRIANA; SANFELICE, RAFAELA C.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Electronic Tongue Based on Nanostructured Hybrid Films of Gold Nanoparticles and Phthalocyanines for Milk Analysis. Journal of Nanomaterials, 2015. (14/16789-5, 13/26712-7, 12/23880-3)

PAVINATTO, ADRIANA; MERCANTE, LUIZA A.; FACURE, MURILO H. M.; PENA, RAFAELLA B.; SANFELICE, RAFAELA C.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Ultrasensitive biosensor based on polyvinylpyrrolidone/chitosan/reduced graphene oxide electrospun nanofibers for 17 alpha - Ethinylestradiol electrochemical detection. Applied Surface Science, v. 458, p. 431-437, NOV 15 2018. (17/19470-8, 17/12174-4, 13/26712-7)

PAVINATTO, ADRIANA; MERCANTE, LUIZA A.; LEANDRO, CLEITON S.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Layer-by-Layer assembled films of chitosan and multi-walled carbon nanotubes for the electrochemical detection of 17 alpha-ethinylestradiol. Journal of Electroanalytical Chemistry, v. 755, p. 215-220, OCT 15 2015. (14/16789-5, 13/26712-7, 12/23880-3)

ANDRE, RAFAELA S.; PAVINATTO, ADRIANA; MERCANTE, LUIZA A.; PARIS, ELAINE C.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Improving the electrochemical properties of polyamide 6/polyaniline electrospun nanofibers by surface modification with ZnO nanoparticles. RSC ADVANCES, v. 5, n. 90, p. 73875-73881, 2015. (14/16789-5, 13/26712-7, 12/23880-3)

MIGLIORINI, FERNANDA L.; SANFELICE, RAFAELA C.; PAVINATTO, ADRIANA; STEFFENS, JULIANA; STEFFENS, CLARICE; CORREA, DANIEL S.. Voltammetric cadmium(II) sensor based on a fluorine doped tin oxide electrode modified with polyamide 6/chitosan electrospun nanofibers and gold nanoparticles. Microchimica Acta, v. 184, n. 4, p. 1077-1084, APR 2017. (13/26712-7, 14/16789-5)

PAVINATTO, ADRIANA; MERCANTE, LUIZA A.; FACURE, MURILO H. M.; PENA, RAFAELLA B.; SANFELICE, RAFAELA C.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Ultrasensitive biosensor based on polyvinylpyrrolidone/chitosan/reduced graphene oxide electrospun nanofibers for 17α - Ethinylestradiol electrochemical detection. Applied Surface Science, v. 458, p. 7-pg., 2018-11-15. (17/19470-8, 17/12174-4, 13/26712-7)

PAVINATTO, ADRIANA; MERCANTE, LUIZA A.; LEANDRO, CLEITON S.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Layer-by-Layer assembled films of chitosan and multi-walled carbon nanotubes for the electrochemical detection of 17 alpha-ethinylestradiol. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 755, p. 6-pg., 2015-10-15. (14/16789-5, 13/26712-7, 12/23880-3)

MERCANTE, LUIZA A.; SCAGION, VANESSA P.; PAVINATTO, ADRIANA; SANFELICE, RAFAELA C.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Electronic Tongue Based on Nanostructured Hybrid Films of Gold Nanoparticles and Phthalocyanines for Milk Analysis. Journal of Nanomaterials, v. 2015, p. 7-pg., 2015-01-01. (14/16789-5, 12/23880-3, 13/26712-7)

SANFELICE, RAFAELA C.; PAVINATTO, ADRIANA; GONCALVES, VANESSA C.; CORREA, DANIEL S.; MATTOSO, LUIZ H. C.; BALOGH, DEBORA T.. Synthesis of a Nanocomposite Containing a Water-Soluble Polythiophene Derivative and Gold Nanoparticles. JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, v. 54, n. 13, p. 1245-1254, JUL 1 2016. (10/11040-5, 14/16789-5, 13/26712-7)

SANFELICE, RAFAELA C.; MERCANTE, LUIZA A.; PAVINATTO, ADRIANA; TOMAZIO, NATHALIA B.; MENDONCA, CLEBER R.; RIBEIRO, SIDNEY J. L.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Hybrid composite material based on polythiophene derivative nanofibers modified with gold nanoparticles for optoelectronics applications. Journal of Materials Science, v. 52, n. 4, p. 1919-1929, FEB 2017. (14/16789-5, 13/26712-7, 12/23880-3)

MERCANTE, LUIZA A.; PAVINATTO, ADRIANA; IWAKI, LEONARDO E. O.; SCAGION, VANESSA P.; ZUCOLOTTO, VALTENCIR; OLIVEIRA, JR., OSVALDO N.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Electrospun Polyamide 6/Poly(allylamine hydrochloride) Nanofibers Functionalized with Carbon Nanotubes for Electrochemical Detection of Dopamine. ACS APPLIED MATERIALS & INTERFACES, v. 7, n. 8, p. 4784-4790, MAR 4 2015. (14/16789-5, 13/26712-7, 12/23880-3)