Smart surfaces: nanostructured and bioinspired systems for the investigation of molecular interactions in membrane models and for the production of devices for biosensors and environmental analysis

• Grant number: 15/23446-0
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2016 - August 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Luciano Caseli
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

In this project, we intended to incorporate bioactive materials, synthetic or natural, on nanostructured bioinspired surfaces, including Langmuir monolayers, Langmuir-Blodgett films and layer-by-layer films. Some candidates are those with high pharmacological interest, associated with events that occur in cell membranes, either by simply destroying the cell membrane as for antimicrobial and antifungal activities, or by acting on cellular mechanisms associated with biochemical events such as signaling and cellular transport, characterizing their expression in microbes and tumor cells. Also, we intended to immobilize enzymes, antibodies and pieces of nucleic acids in nanostructured films to investigate the properties of these substances in terms of molecular recognition, and the possibility of incorporating them in a bioinspired system. For that, substances that assist in the molecular accommodation increasing the biological activity can be studied. Some candidates are polysaccharides and carbon nanotubes. In this sense, we emphasize the strong interaction that the applicant already has with groups within the institution, specialists in obtaining bioactive materials, and experts in molecular biology. This project therefore aims at studying smart surfaces constructed from bioinspired interfaces organized at the molecular level. (AU)

Scientific publications

(24)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, JOAO VICTOR N.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Thymol in cellular membrane models formed by negative charged lipids causes aggregation at the air-water interface. Chemical Physics Letters, v. 717, p. 87-90, FEB 16 2019. (15/23446-0, 13/14262-7)

FERREIRA, JOAO VICTOR N.; CAPELLO, TABATA M.; SIQUEIRA, LEONARDO J. A.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Mechanism of Action of Thymol on Cell Membranes Investigated through Lipid Langmuir Monolayers at the Air-Water Interface and Molecular Simulation. Langmuir, v. 32, n. 13, p. 3234-3241, APR 5 2016. (15/23446-0, 13/10213-1)

TELES ROSSINI, CABO VINICIUS; MOLINA, CELSO; CASELI, LUCIANO. Langmuir and Langmuir-Blodgett films of di-ureasil hybrid compounds containing phosphotungstic acid. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 524, p. 35-42, JUL 5 2017. (15/23446-0, 13/14262-7, 14/12557-2)

GROSSO SALIS, LUIZ FERNANDO; JAROQUE, GUILHERME NUNEZ; BERRIO ESCOBAR, JHON FERNANDO; GIORDANI, CRISTIANO; MARTINEZ MARTINEZ, ALEJANDRO; MARQUEZ FERNANDEZ, DIANA MARGARITA; CASTELLI, FRANCESCO; SARPIETRO, MARIA GRAZIA; CASELI, LUCIANO. Interaction of 3 ',4 ',6 '-trimyristoyl-uridine derivative as potential anticancer drug with phospholipids of tumorigenic and non-tumorigenic cells. Applied Surface Science, v. 426, p. 10-pg., 2017-12-31. (15/23446-0)

DE SOUZA, KARINE DAMACENO; PEREZ, KATIA REGINA; DURAN, NELSON; JUSTO, GISELLE ZENKER; CASELI, LUCIANO. Interaction of violacein in models for cellular membranes: Regulation of the interaction by the lipid composition at the air-water interface. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 160, p. 247-253, DEC 1 2017. (15/23446-0)

AYOUB, FABIO DE PAULA; CASELI, LUCIANO. Controlling the molecular architecture of lactase immobilized in Langmuir-Blodgett films of phospholipids to modulate the enzyme activity. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 150, p. 8-14, FEB 1 2017. (15/23446-0, 14/12557-2)

RODRIGUES, JEFFERSON CARNEVALLE; CASELI, LUCIANO. Incorporation of bacitracin in Langmuir films of phospholipids at the air-water interface. Thin Solid Films, v. 622, p. 95-103, JAN 31 2017. (15/23446-0)

GOTO, THIAGO E.; LOPES, CARLA C.; NADER, HELENA B.; SILVA, ANIELLE C. A.; DANTAS, NOELI O.; SIQUEIRA, JOSE R., JR.; CASELI, LUCIANO. CdSe magic-sized quantum dots incorporated in biomembrane models at the air-water interface composed of components of tumorigenic and non-tumorigenic cells. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1858, n. 7, p. 8-pg., 2016-07-01. (15/23446-0)

ARAUJO, FELIPE TEJADA; PERES, LAURA OLIVEIRA; CASELI, LUCIANO. Conjugated Polymers Blended with Lipids and Galactosidase as Langmuir-Blodgett Films To Control the Biosensing Properties of Nanostructured Surfaces. Langmuir, v. 35, n. 22, p. 7294-7303, JUN 4 2019. (19/03239-0, 14/50869-6, 13/14262-7, 15/23446-0)

JAROQUE, GUILHERME NUNEZ; SARTORELLI, PATRICIA; CASELI, LUCIANO. Interfacial vibrational spectroscopy and Brewster angle microscopy distinguishing the interaction of terpineol in cell membrane models at the air-water interface. Biophysical Chemistry, v. 246, p. 1-7, MAR 2019. (15/23446-0, 13/14262-7)

GUIMARAES GONCALVES, GIULIA ELISA; MORAIS, THIAGO RAHAL; GOMES, KAIO DE SOUZA; COSTA-SILVA, THAIS ALVES; TEMPONE, ANDRE GUSTAVO; GHILARDI LAGO, JOAO HENRIQUE; CASELI, LUCIANO. Antitrypanosomal activity of epi-polygodial from Drimys brasiliensis and its effects in cellular membrane models at the air-water interface. BIOORGANIC CHEMISTRY, v. 84, p. 186-191, MAR 2019. (15/23446-0, 13/14262-7, 18/07885-1, 15/23403-9)

BARBOSA, CAMILA GOUVEIA; LA-SCALEA, MAURO AQUILES; CASELI, LUCIANO; PERES, LAURA OLIVEIRA. Copolymers and enzymes blended as LB films changing the bioelectronics properties of interfaces. COLLOID AND INTERFACE SCIENCE COMMUNICATIONS, v. 27, p. 40-44, NOV 2018. (14/50869-6, 15/23446-0)

DE BRITO, AUDREY K.; NORDI, CRISTINA S. F.; CASELI, LUCIANO. Polarization Modulation Reflection-Absorption Spectroscopy applied in ultrathin films of algal biomacromolecules may explain the mechanism associated to the removal of pollutant metals. VIBRATIONAL SPECTROSCOPY, v. 92, p. 9-13, SEP 2017. (15/23446-0)

BARBOSA, CAMILA GOUVEIA; CASELI, LUCIANO; PERES, LAURA OLIVEIRA. Conjugated polymers nanostructured as smart interfaces for controlling the catalytic properties of enzymes. Journal of Colloid and Interface Science, v. 476, p. 206-213, AUG 15 2016. (15/23446-0)

GOTO, THIAGO E.; LOPES, CARLA C.; NADER, HELENA B.; SILVA, ANIELLE C. A.; DANTAS, NOELI O.; SIQUEIRA, JR., JOSE R.; CASELI, LUCIANO. CdSe magic-sized quantum dots incorporated in biomembrane models at the air-water interface composed of components of tumorigenic and non-tumorigenic cells. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1858, n. 7, A, p. 1533-1540, JUL 2016. (15/23446-0)

ROCHA, JEFFERSON M.; PAVINATTO, ADRIANA; NOBRE, THATYANE M.; CASELI, LUCIANO. Acylated Carrageenan Changes the Physicochemical Properties of Mixed Enzyme-Lipid Ultrathin Films and Enhances the Catalytic Properties of Sucrose Phosphorylase Nanostructured as Smart Surfaces. Journal of Physical Chemistry B, v. 120, n. 24, p. 5359-5366, JUN 23 2016. (15/23446-0)

DA ROCHA JUNIOR, CARLOS; CASELI, LUCIANO. Adsorption and enzyme activity of asparaginase at lipid Langmuir and Langmuir-Blodgett films. Materials Science & Engineering C-Materials for Biological Applications, v. 73, p. 579-584, APR 1 2017. (15/23446-0, 14/12557-2, 15/10446-1)

RODRIGUES, RAUL T.; MORAIS, PAULO V.; NORDI, CRISTINA S. F.; SCHOENING, MICHAEL J.; SIQUEIRA, JR., JOSE R.; CASELI, LUCIANO. Carbon Nanotubes and Algal Polysaccharides To Enhance the Enzymatic Properties of Urease in Lipid Langmuir-Blodgett Films. Langmuir, v. 34, n. 9, p. 3082-3093, MAR 6 2018. (15/23446-0, 16/01867-6)

RODRIGUES, JEFFERSON CARNEVALLE; CASELI, LUCIANO. Lipids mediating the interaction of metronidazole with cell membrane models at the air-water interface. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 171, p. 377-382, NOV 1 2018. (15/23446-0, 13/14262-7)

GROSSO SALIS, LUIZ FERNANDO; JAROQUE, GUILHERME NUNEZ; BERRIO ESCOBAR, JHON FERNANDO; GIORDANI, CRISTIANO; MARTINEZ MARTINEZ, ALEJANDRO; MARQUEZ FERNANDEZ, DIANA MARGARITA; CASTELLI, FRANCESCO; SARPIETRO, MARIA GRAZIA; CASELI, LUCIANO. Interaction of 3 `,4 `,6 `-trimyristoyl-uridine derivative as potential anticancer drug with phospholipids of tumorigenic and non-tumorigenic cells. Applied Surface Science, v. 426, p. 77-86, DEC 31 2017. (15/23446-0)

SORIANO, GUSTAVO BONOMI; OLIVEIRA, ROSELAINE DA SILVA; CAMILO, FERNANDA FERRAZ; CASELI, LUCIANO. Interaction of non-aqueous dispersions of silver nanoparticles with cellular membrane models. Journal of Colloid and Interface Science, v. 496, p. 111-117, JUN 15 2017. (14/23065-3, 15/23446-0)

GONCALVES, GIULIA ELISA G.; MORAIS, THIAGO R.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Incorporation of polygodial in Langmuir films of selected lipids. Thin Solid Films, v. 669, p. 19-28, JAN 1 2019. (15/23446-0, 18/07885-1)

TELES ROSSINI, CAIO VINICIUS; MOLINA, CELSO; CASELI, LUCIANO. Immobilization of urease in Langmuir-Blodgett films of di-ureasil hybrid compounds. Thin Solid Films, v. 670, p. 17-23, JAN 31 2019. (15/23446-0, 13/14262-7, 14/12557-2)

SAKAI, ANDREI; DE SOUSA MESQUITA, ANA PAULA; DE CASTRO LEVATTI, ERICA VALADARES; STRAUS, ANITA HILDA; NADER, HELENA B.; LOPES, CARLA CRISTINA; CASELI, LUCIANO. Interaction of Trastuzumab with biomembrane models at air-water interfaces mimicking cancer cell surfaces. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1861, n. 10, OCT 1 2019. (19/03239-0, 15/23446-0, 13/14262-7)