Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices

• Grant number: 22/03736-7
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2022
• End date: March 31, 2025
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Luciano Caseli
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Celso Molina ; Thaís Fernandes Schmidt
• Associated research grant(s): 22/13938-6 - Nanostructured materials based on alkylated nucleosides with anticancer properties incorporated in Langmuir and Langmuir-Blodgett films of lipids and luminescent probes, AP.R SPRINT

Abstract

In this project, we intend to use Langmuir and Langmuir-Blodgett (LB) films with two main objectives: (i) interaction studies of bioactive compounds in biointerface models, including cell membranes, viral envelopes, alveolar membranes, and supramolecular lipoprotein systems, and (ii) for the immobilization of enzymes, carbonaceous materials, and conductive polymers in ultra-thin nanostructured systems for the manufacture of bioinspired optoelectronic devices. Langmuir films will be produced from insoluble amphiphilic materials, such as lipids and polymers, and conveniently mixed with proteins, enzymes, and compounds with potential bioactivity, including those with antimicrobial, antiviral properties, antifungal, or antitumor activity. These materials include metallic nanoparticles and organic-inorganic hybrid materials, which will interact at the interface and be investigated through tensiometric, electrical, rheological, spectroscopic, and morphological measurements. Furthermore, we intend to immobilize enzymes, antibodies, and pieces of nucleic acids on LB films to produce optoelectronic devices capable of molecular recognition. For this, substances that help in molecular accommodation will be used, modulating the biological activity and the response signal induced by the interaction. Among the candidates are conductive polymers, metallic nanoparticles, polysaccharides, and carbonaceous materials (graphene and carbon nanotubes). In this sense, seek understanding at the molecular level about the interactions that help in the understanding of biophysical and biochemical processes in biointerfaces, many of them related to infectious processes or cell proliferation, as well as assist in the construction of intelligent surfaces built from organized bioinspired interfaces at the molecular level for the fabrication of optoelectronic devices of interest. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NERATH, GABRIEL; OLIVEIRA, DANILO A.; SIQUEIRA, JOSE R.; CASELI, LUCIANO. Using Carbon Nanotubes to Improve Enzyme Activity and Electroactivity of Fatty Acid Langmuir-Blodgett Film-Incorporated Galactose Oxidase for Sensing and Energy Storage Applications. ACS APPLIED MATERIALS & INTERFACES, v. N/A, p. 11-pg., 2024-02-09. (18/22214-6, 22/03736-7)

SANTANA-LIMA, BEATRIZ; BELAUNDE, LUCAS HUMBERTO ZIMMERMANN; DE SOUZA, KARINE DAMACENO; ROSA, MATHEUS ELIAS; DE CARVALHO, JOSE EDUARDO; MACHADO-JR, JOEL; ALONSO-VALE, MARIA ISABEL CARDOSO; CASELI, LUCIANO; RANDO, DANIELA GONCALES GALASSE; CAPERUTO, LUCIANA CHAGAS. Acute Kaempferol Stimulation Induces AKT Phosphorylation in HepG2 Cells. LIFE-BASEL, v. 14, n. 6, p. 17-pg., 2024-06-01. (19/26686-2, 19/13618-9, 22/03736-7, 20/12962-5)

DE SOUZA, MATHEUS LIMA; MACHADO, ANDRE CAMPOS; BARBOSA, HENRIQUE; LAGO, JOAO HENRIQUE GHILARDI; CASELI, LUCIANO. Interaction of sakuranetin with unsaturated lipids forming Langmuir monolayers at the air-water interface: A biomembrane model. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 234, p. 7-pg., 2024-01-13. (22/04241-1, 22/09202-4, 22/03736-7)

LONDERO, VINICIUS S.; ROSA, MATHEUS E.; BAITELLO, JOAO B.; COSTA-SILVA, THAIS A.; CRUZ, LUCAS MONTEIRO S.; TEMPONE, ANDRE G.; CASELI, LUCIANO; LAGO, JOAO HENRIQUE G.. Barbellatanic acid, a new antitrypanosomal pseudo-disesquiterpenoid isolated from Nectandra barbellata, displayed interaction with protozoan cell membrane. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1865, n. 7, p. 9-pg., 2023-10-01. (22/03736-7, 18/22214-6, 14/50869-6, 21/02789-7, 21/15084-1, 17/17044-1)

MACHADO, A. C.; SALVO-COMINO, C.; RODRIGUEZ-MENDEZ, M. L.; CASELI, L.. Enhancing biosensor capabilities through laccase immobilization in nanostructured Langmuir-Blodgett films for phenol detection. RESULTS IN SURFACES AND INTERFACES, v. 18, p. 9-pg., 2024-11-27. (22/03736-7, 18/22214-6, 22/04241-1, 23/00329-4)

GONCALVES, GIULIA ELISA G.; OLIVEIRA, SAMUEL; GOMES, KAIO DE SOUZA; COSTA-SILVA, THAIS ALVES; TEMPONE, ANDRE GUSTAVO; LAGO, JOAO HENRIQUE GHILARDI; CASELI, LUCIANO. Effect of partial O-methylation in dehydrodieugenol on its antitrypanosomal activity-correlation with the toxicity using cell membrane models. Biophysical Chemistry, v. 296, p. 11-pg., 2023-02-24. (19/03239-0, 21/02789-7, 22/03736-7)

PIRES, GUILHERME HENRIQUE DA CRUZ RAMOS; BARBOSA, HENRIQUE; ALMEIDA, ROBERTO BAPTISTA PEREIRA; LAGO, JOAO HENRIQUE GHILARDI; CASELI, LUCIANO. Ethanolamine phospholipids at the air-water interface as cell membranes models of microorganisms to study the nanotoxicology of sakuranetin. Thin Solid Films, v. 770, p. 8-pg., 2023-03-01. (19/03239-0, 19/18125-0, 18/22214-6, 21/02789-7, 22/03736-7)

MACHADO, ANDRE CAMPOS; DA SILVA, TAMIRIS REISSA CIPRIANO; RAMINELLI, CRISTIANO; CASELI, LUCIANO. The composition of fusogenic lipid mixtures at the air-water modulates the physicochemical properties changes upon interaction with lysicamine. Biophysical Chemistry, v. 293, p. 8-pg., 2022-12-23. (20/01183-5, 20/12530-8, 18/22214-6, 22/03736-7)

FERNANDES, NATHALIA MARIA MORAES; CASELI, LUCIANO; BAGATIN, IZILDA A.. Bioinspired nanoarchitectonics at the air-water interface to understand the interaction of lipids with a Europium-coordinated quinoline derivative. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 229, p. 6-pg., 2023-09-01. (15/20485-4, 22/03736-7, 18/22214-6)

SCHOLL, FABIO A.; OLIVEIRA, DANILO A.; SIQUEIRA JR, JOSE R. SIQUEIRA; CASELI, LUCIANO. Exploring Langmuir-Blodgett films with phospholipid-graphene oxide/MnO2 as a hybrid nanostructured interface for supercapacitor applications. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 664, p. 6-pg., 2023-02-24. (18/22214-6, 22/03736-7, 14/50869-6)

ROSA, MATHEUS ELIAS; BRITO, IVANILDO A.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Exploring the influence of acetylenic acetogenin unsaturation patterns on lipid interface interactions: Insights from surface chemistry, rheology, and spectroscopy. RESULTS IN SURFACES AND INTERFACES, v. 17, p. 11-pg., 2024-09-25. (22/03736-7, 18/22214-6, 21/15084-1)

MOREIRA, FELIPE ALMEIDA; ESCOBAR, JHON FERNANDO BERRIO; GIORDANI, CRISTIANO; CASELI, LUCIANO. Exploring the physicochemical properties of the integration of Tristearoyl uridine in Langmuir monolayers: An approach to cell membrane modeling for prodrugs. Biophysical Chemistry, v. 310, p. 9-pg., 2024-05-09. (23/00850-6, 22/03736-7, 22/13938-6)

MOREIRA, FELIPE ALMEIDA; ESCOBAR, JHON FERNANDO BERRIO; GIORDANI, CRISTIANO; CASELI, LUCIANO. Langmuir monolayers provide an effective strategy for studying molecular recognition of nucleobases using alkylated nucleotides. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 244, p. 8-pg., 2024-08-08. (23/00850-6, 22/03736-7, 22/13938-6)

DA SILVA, GUSTAVO H. O.; DOS SANTOS, KEVIN F.; BARCELLOS, ALINE F.; DE SOUSA, RAQUEL M. FERREIRA; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Exploring the selective incorporation of 15β-senecioyloxi-ent-kaurenoic acid methyl ester in Langmuir monolayers mimicking cell membranes. BIOORGANIC CHEMISTRY, v. 153, p. 12-pg., 2024-12-01. (18/22214-6, 23/12447-1, 22/03736-7)

DE SOUZA, MATHEUS LIMA; MACHADO, ANDRE CAMPOS; BARBOSA, HENRIQUE; PEREIRA ALMEIDA, ROBERTO BAPTISTA; CASELI, LUCIANO. Thermodynamic, rheological, and morphological properties of sakuranetin incorporated in langmuir monolayers of unsaturated lipids as a biomembrane model. BIOPHYSICAL JOURNAL, v. 123, n. 3, p. 1-pg., 2024-02-08. (22/03736-7)

JAROQUE, GUILHERME NUNEZ; DOS SANTOS, AUGUSTO LEONARDO; SARTORELLI, PATRICIA; CASELI, LUCIANO. Unsaturation of serine lipids modulating the interaction of a cytosporone with models of the external leaflet of tumorigenic cell membranes. Chemistry and Physics of Lipids, v. 258, p. 8-pg., 2023-12-09. (22/09202-4, 22/03736-7)