Langmuir and Langmuir-Blodgett films of lipids containing phenylalanine dehydrogenase for sensors of phenylalanine

• Grant number: 18/00291-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date until: April 01, 2018
• End date until: July 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Luciano Caseli
• Grantee: Rafael Leonardo Cruz Gomes da Silva
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated research grant: 13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM
• Associated scholarship(s): 19/01797-6 - Langmuir and Langmuir-Blodgett films of lipids containing phenylalanine dehydrogenase for phenylalanine biosensors, BE.EP.MS

Abstract

In this project, we intend to study the immobilization of the enzyme phenylalanine dehydrogenase (PNH) in Langmuir and Langmuir-Blodgett (LB) lipid films, as well as to investigate their physicochemical properties and possible technological applications of the nanostructured systems produced. These properties include surface measurements on the Langmuir monolayers (tensiometry, surface potential, Brewster angle microscopy, vibrational spectroscopy and rheological measurements), characterization of the LB films by UV-vis and infrared spectroscopies, atomic force microscopy and nanogravimmetry by quartz crystal microbalance, as well as viability of LB films produced for phenylalanine sensors through the estimation of enzymatic activity by colorimetric methods, and electrical capacitance measurements for the assembly of analytical curves. It is intended not only to use mixed lipid-enzyme systems, but also other substances capable of improve the molecular accommodation of the enzyme, as well as to enhance the optical and electrical signal of the systems produced. This will include the introduction of carbon nanotubes, polysaccharides, metal nanoparticles, and conductive polymers. The results will be evaluated from the perspective of possible implications in nanobiotechnology, inferred from the effects caused by carbon nanotubes, polysaccharides, and conductive polymers in the artificial membranes, especially with the incorporation of enzymes. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRUZ GOMES DA SILVA, RAFAEL LEONARDO; SHARMA, SHIV K.; PAUDYAL, SURAJ; MINTZ, KEENAN J.; LEBLANC, ROGER M.; CASELI, LUCIANO. Surface Chemistry Studies on the Formation of Mixed Stearic Acid/Phenylalanine Dehydrogenase Langmuir and Langmuir-Blodgett Films. Langmuir, v. 37, n. 25, p. 7771-7779, JUN 29 2021. (19/01797-6, 18/22214-6, 18/00291-9, 19/03239-0)

RODRIGUES, REBECA DA ROCHA; CRUZ GOMES DA SILVA, RAFAEL LEONARDO; CASELI, LUCIANO; PERES, LAURA OLIVEIRA. Conjugated polymers as Langmuir and Langmuir-Blodgett films: Challenges and applications in nanostructured devices. ADVANCES IN COLLOID AND INTERFACE SCIENCE, v. 285, NOV 2020. (18/00291-9, 14/50869-6, 19/03239-0)

BARBOSA, HENRIQUE; DA SILVA, RAFAEL LEONARDO C. G.; COSTA-SILVA, THAIS A.; TEMPONE, ANDRE G.; ANTAR, GUILHERME M.; LAGO, JOAO HENRIQUE G.; CASELI, LUCIANO. Interaction of dicentrinone, an antitrypanosomal aporphine alkaloid isolated from Ocotea puberula (Lauraceae), in cell membrane models at the air-water interface. BIOORGANIC CHEMISTRY, v. 101, AUG 2020. (18/00291-9, 18/10279-6, 19/03239-0, 18/07885-1)

DA SILVA, RAFAEL LEONARDO C. G.; GONCALVES, KARINA DE OLIVEIRA; COURROL, LILIA CORONATO; CASELI, LUCIANO. Study of the interactions of gold nanoparticles functionalized with aminolevulinic acid in membrane models. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 205, SEP 2021. (19/03239-0, 18/00291-9, 17/23686-6, 18/22214-6)