Interaction of the enzyme asparaginase in phospholipid Langmuir and Langmuir-Blodgett films: possible application for biosensors of asparagine

• Grant number: 15/10446-1
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2015
• Effective date (End): January 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal researcher: Luciano Caseli
• Grantee: Carlos da Rocha Junior
• Home Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

This work is intended to study the interaction of the enzyme asparaginase in Langmuir and Langmuir-Blodgett (LB) films of dipalmitoylphosphatidylcholine (DPPC). The phospholipid will be spread on the air-water interface and the enzyme inserted into the aqueous subphase of the lipid monolayer. The adsorption of the enzyme to the monolayer will be studied with surface pressure-area isotherms, vibrational spectroscopy and Brewster angle microscopy. The mixed monolayer will be transferred to solid supports by the LB methodology and the enzymatic activity of asparaginase will be evaluated from solutions containing asparagine. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA ROCHA JUNIOR, CARLOS; CASELI, LUCIANO. Adsorption and enzyme activity of asparaginase at lipid Langmuir and Langmuir-Blodgett films. Materials Science & Engineering C-Materials for Biological Applications, v. 73, p. 579-584, APR 1 2017. (15/23446-0, 15/10446-1, 14/12557-2)