Interaction of natural acetogenins with biological activity in membrane models at the air-water interface

• Grant number: 21/15084-1
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): August 01, 2022
• Status: Discontinued
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Luciano Caseli
• Grantee: Matheus Elias Rosa
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 23/16020-2 - Studying the interaction of antiprotozoal acetogenins with membrane models utilizing mixed Langmuir films of lipids and mucins, BE.EP.DD

Abstract

In this project, we intend to study the action of acetogenins isolated from the plant species Porcelia macrocarpa (Annonaceae) and which showed antiparasitic action, in cell membrane models in the form of Langmuir and Langmuir-Blodgett (LB) films of lipids, and complemented with studies in liposomes and through molecular simulation. The lipids to be used will be those found in cell membranes (phosphatidylcholines, phosphatidylglycerols, phosphatidylethanolamines, phosphatidylserines, sphingolipids and cholesterol), which will be spread at the air-water interface forming Langmuir films. The incorporation of compounds into Langmuir films will be evaluated through pressure and surface potential measurements, adsorption kinetic curves, infrared absorption-reflection spectroscopy (PM-IRRAS), Brewster Angle Microscopy (BAM) and interfacial rheology dilatational and by shear. The floating monolayers will be transferred to solid supports by the LB technique and characterized by Atomic Force Microscopy (AFM), fluorescence spectroscopy, PM-IRRAS and quartz crystal microbalance nanogravimetry. In front of liposomes, the action of the compound incorporated in unilamellar vesicles interacting with Langmuir films will be investigated. The results will be evaluated from the perspective of possible biological implications, inferred from the effects caused by the compound on artificial membranes in search of an understanding of the mechanism at the molecular level for the prodrug-membrane interaction. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LONDERO, VINICIUS S.; ROSA, MATHEUS E.; BAITELLO, JOAO B.; COSTA-SILVA, THAIS A.; CRUZ, LUCAS MONTEIRO S.; TEMPONE, ANDRE G.; CASELI, LUCIANO; LAGO, JOAO HENRIQUE G.. Barbellatanic acid, a new antitrypanosomal pseudo-disesquiterpenoid isolated from Nectandra barbellata, displayed interaction with protozoan cell membrane. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1865, n. 7, p. 9-pg., 2023-10-01. (22/03736-7, 18/22214-6, 14/50869-6, 21/02789-7, 21/15084-1, 17/17044-1)