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Interaction of natural acetogenins with biological activity in membrane models at the air-water interface

Grant number: 21/15084-1
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): August 01, 2022
Effective date (End): July 31, 2027
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal Investigator:Luciano Caseli
Grantee:Matheus Elias Rosa
Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
Associated research grant:18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM


In this project, we intend to study the action of acetogenins isolated from the plant species Porcelia macrocarpa (Annonaceae) and which showed antiparasitic action, in cell membrane models in the form of Langmuir and Langmuir-Blodgett (LB) films of lipids, and complemented with studies in liposomes and through molecular simulation. The lipids to be used will be those found in cell membranes (phosphatidylcholines, phosphatidylglycerols, phosphatidylethanolamines, phosphatidylserines, sphingolipids and cholesterol), which will be spread at the air-water interface forming Langmuir films. The incorporation of compounds into Langmuir films will be evaluated through pressure and surface potential measurements, adsorption kinetic curves, infrared absorption-reflection spectroscopy (PM-IRRAS), Brewster Angle Microscopy (BAM) and interfacial rheology dilatational and by shear. The floating monolayers will be transferred to solid supports by the LB technique and characterized by Atomic Force Microscopy (AFM), fluorescence spectroscopy, PM-IRRAS and quartz crystal microbalance nanogravimetry. In front of liposomes, the action of the compound incorporated in unilamellar vesicles interacting with Langmuir films will be investigated. The results will be evaluated from the perspective of possible biological implications, inferred from the effects caused by the compound on artificial membranes in search of an understanding of the mechanism at the molecular level for the prodrug-membrane interaction. (AU)

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