| Full text | |
| Author(s): |
Rodrigues, Valquiria C.
[1]
;
Moraes, Marli L.
[2]
;
Soares, Juliana C.
[1]
;
Soares, Andrey C.
[1]
;
Sanfelice, Rafaela
[3]
;
Deffune, Elenice
[4]
;
Oliveira Jr, Osvaldo N.
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Paulo, Unifesp, Campus Sao Jose dos Campos, Sao Jose Dos Campos, SP - Brazil
[3] Univ Fed Triangulo Mineiro, Dept Chem Engn, Uberaba, MG - Brazil
[4] UNESP, Dept Urol, Med Sch, Botucatu, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN; v. 91, n. 6, p. 891-896, JUN 2018. |
| Web of Science Citations: | 15 |
| Abstract | |
We report on immunosensors to detect D-dimer, a biomarker of venous thromboembolism, which are made with layer-by-layer (LbL) films containing immobilized anti-D-dimer monoclonal antibody alternated with a layer of chitosan/gold nanoparticles (AuNpChi). Detection was due to irreversible adsorption of the antigen D-dimer on its corresponding antibody according to a Langmuir-Freundlich model, thus giving rise to ellipsoidal structures in scanning electron microscopy images whose size and number increased with D-dimer concentration. The chemical groups involved in the adsorption process were inferred from polarization-modulated infrared reflection absorption (PM-IRRAS) through changes in the amide and carbonyl bands. Detection of D-dimer was made with electrical impedance spectroscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The latter was the most sensitive with a detection limit of 9 x 10(-4) mu g/mL, sensitivity of 0.27 x 10(-6) A/mu gmL(-1) with linear increase from 0 to 1 mu g/mL. The selectivity of the immunosensor made with AuNpChi/anti-D-dimer film was verified by noting negligible changes in the cyclic voltammograms when exposed to typical interferents in biological fluids. (AU) | |
| FAPESP's process: | 13/14262-7 - Nanostructured films from biologically-relevant materials |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |