Immunosensor for the diagnosis of venous thromboembolism based on immobilization of the antibody on silk fibroin

• Grant number: 14/03145-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2014
• End date: December 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Marli Leite de Moraes
• Grantee: Anna Laura Yuri Yokomichi
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The project to be developed by the student is part of a broader project that aims at the development of immunosensors from monoclonal antibodies immobilized on polymeric matrices in nanostructured films (Project Universal 480896/2013-5). The proposal is based on the immobilization of monoclonal antibody anti-FDP (fibrin degradation product) on silk fibroin in LbL films for the diagnosis of venous thromboembolism. Initially will be necessary to standardize the method of immobilization of the biomolecules and investigate the molecular mechanisms involved in the interaction between the silk fibroin and anti-PDF. These studies will be conducted using spectroscopic techniques, UV-visible and fluorescence. The response of the immunosensor will be investigated by antigen-antibody interaction, i. e. the D-dimer, which is the fibrin degradation productf, and anti-FDP, which will be monitored with measurements of electrical impedance and/or electrochemical.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YOKOMICHI, ANNA LAURA YURI; RODRIGUES, VALQUIRIA DA CRUZ; MOROZ, ANDREI; BERTANHA, MATHEUS; RIBEIRO, SIDNEY J. L.; DEFFUNE, ELENICE; MORAES, MARLI L.. Detection of factor VIII and D-dimer biomarkers for venous thromboembolism diagnosis using electrochemistry immunosensor. Talanta, v. 219, NOV 1 2020. (14/03145-2)