Printed and implantable biosensor made from biopolymers for long-term monitoring

• Grant number: 16/06612-6
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): June 01, 2016
• Effective date (End): October 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Osvaldo Novais de Oliveira Junior
• Grantee: Robson Rosa da Silva
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM

Abstract

Implantable biosensors are still limited with regard to long-term monitoring of biological functions, and there is no report in the literature of their use for months or more. The main reason for this limitation is that biosensors are not fully biocompatible or they are bioabsorbed by the biological tissues. In this Project, we aim at fabricating flexible enzymatic biosensors that may be implantable for long-term monitoring (of the order of months), using ink-jet printing. The biosensors will be made with the natural polymers silk fibroin and bacterial cellulose, with horseradish peroxidase (HRP) serving as prototypical enzyme. Silk fibroin was selected because it increases enzyme lifetime, and it will be used in aqueous solutions for preparing the flexible supports as well as in the ink to print the enzyme-containing layer. The principle of detection for biosensing will be impedance spectroscopy, with an interdigitated architecture printed with fibroin ink containing conducting nanoparticles of Au and/or Ag. The final device will be coated with a layer of bacterial cellulose hydrogel, which is biocompatible and is not bioabsorbed rapidly by biological tissues. The combination of natural polymers and ink-jet printing may allow for the fabrication of flexible, implantable biosensors that may last for months and be potentially of low cost. Owing to the duration of the Project, in vivo tests are not planned, for which partnerships will be sought on the course of the research.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARAUJO, INES M. S.; SILVA, ROBSON R.; PACHECO, GUILHERME; LUSTRI, WILTON R.; TERCJAK, AGNIESZKA; GUTIERREZ, JUNKAL; JUNIOR, JOSE R. S.; AZEVEDO, FRANCISCO H. C.; FIGUEREDO, GIRLENE S.; VEGA, MARIA L.; et al. Hydrothermal synthesis of bacterial cellulose-copper oxide nanocomposites and evaluation of their antimicrobial activity. Carbohydrate Polymers, v. 179, p. 341-349, JAN 1 2018. (16/06612-6)

SABIO, RAFAEL MIGUEL; DA SILVA, ROBSON ROSA; SARGENTELLI, VAGNER; GUTIERREZ, JUNKAL; TERCJAK, AGNIESZKA; LIMA RIBEIRO, SIDNEY JOSE; BARUD, HERNANE DA SILVA. Growth of magnetic cobalt hexacyanoferrate nanoparticles onto bacterial cellulose nanofibers. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS, v. 30, n. 18, SI, p. 16956-16965, SEP 2019. (16/06612-6)

SILVA, ROBSON R.; RAYMUNDO-PEREIRA, PAULO A.; CAMPOS, ANDERSON M.; WILSON, DEIVY; OTONI, CAIO G.; BARUD, HERNANE S.; COSTA, CARLOS A. R.; DOMENEGUETTI, RAFAEL R.; BALOGH, DEBORA T.; RIBEIRO, SIDNEY J. L.; et al. Microbial nanocellulose adherent to human skin used in electrochemical sensors to detect metal ions and biomarkers in sweat. Talanta, v. 218, OCT 1 2020. (16/01919-6, 13/14262-7, 15/01770-0, 16/06612-6)

REYES GOMEZ, FAUSTINO; RUBIRA, RAFAEL J. G.; CAMACHO, SABRINA A.; MARTIN, CIBELY S.; DA SILVA, ROBSON R.; CONSTANTINO, CARLOS J. L.; ALESSIO, PRISCILA; OLIVEIRA, JR., OSVALDO N.; RICARDO MEJIA-SALAZAR, J.. Surface Plasmon Resonances in Silver Nanostars. SENSORS, v. 18, n. 11, NOV 2018. (16/06612-6, 13/14262-7, 16/09634-0, 16/12311-9, 13/01897-4)

MATURI, FERNANDO E.; SABIO, RAFAEL M.; SILVA, ROBSON R.; LAHOUD, MARCELO G.; MENEGUIN, ANDREIA B.; VALENTE, GUSTAVO T.; CAFACE, RAPHAEL A.; LEITE, ILAIALI S.; INADA, NATALIA M.; RIBEIRO, SIDNEY J. L.. Luminescent Mesoporous Silica Nanohybrid Based on Drug Derivative Terbium Complex. MATERIALS, v. 12, n. 6, MAR 2 2019. (16/06612-6)