Development and characterization of nanostructured films for conductimetric hydrogen peroxide biosensors

• Grant number: 10/50424-3
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2010
• End date: May 31, 2012
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Andrés Vercik
• Grantee: Andrés Vercik
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The aim of this project is to develop chitosan membranes containing metallic nanoparticles and immobilized enzymes, whose conductance depends on the activity of the enzyme for use as sensing element of hydrogen peroxide for glucose biosensors. This kind of material with conductimetric behavior can be introduced in the biasing circuit of MOS field effect transistors, as proposed recently by the author as a novel detection strategy for biosensors. It is expected to study the feasibility of this new type of biosensors, as well as to analyze who the enzymatic activity affects the electronic transport. The chosen enzyme is the HRP (Horse Radish Peroxidase), because of its well known properties and wide use in biochemistry. However, Soy bean peroxidase could be used as a cheaper alternative. The operation principle is the following: the transport in the membranes occurs by tunneling between the nanoparticles; the charge resulting form the redox reaction, catalyzed by the enzyme, can affect the transport via two mechanisms: a) charge transfer between the anzymes and the nanoparticles, occupying bound states within them; b) transfer between the enzymes and the polymeric chains, affecting the molecular bond. In both cases the effects should be sensed in the transport process. This hypothesis must be tested. For this purpose, measurements of conductivity and current-voltage curves as functions of temperature will be performed, which will allow identifying the prevailing process. The chitosan membranes containing the enzymes and nanoparticles are biocompatible, which is desirable for implantable devices. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VALENCIA, GERMAN AYALA; RODRIGUEZ LLANOS, JAIBER HUMBERTO; DE OLIVEIRA VERCIK, LUCI CRISTINA; VERCIK, ANDRES. Electronic transport in organic memories of chitosan with gold nanoparticles. JOURNAL OF POLYMER ENGINEERING, v. 34, n. 2, p. 105-111, APR 2014. (10/50424-3)

VALENCIA, GERMAN AYALA; DE OLIVEIRA VERCIK, LUCI CRISTINA; VERCIK, ANDRES. A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films. JOURNAL OF POLYMER ENGINEERING, v. 34, n. 7, p. 633-638, SEP 2014. (10/50424-3)

VALENCIA, GERMAN AYALA; DE OLIVEIRA VERCIK, LUCI CRISTINA; FERRARI, ROSANA; VERCIK, ANDRES. Synthesis and characterization of silver nanoparticles using water-soluble starch and its antibacterial activity on Staphylococcus aureus. STARCH-STÄRKE, v. 65, n. 11-12, p. 931-937, NOV 2013. (10/50424-3)