|Support type:||Scholarships in Brazil - Post-Doctorate|
|Effective date (Start):||November 01, 2013|
|Effective date (End):||October 31, 2015|
|Field of knowledge:||Physical Sciences and Mathematics - Chemistry - Analytical Chemistry|
|Principal researcher:||Orlando Fatibello Filho|
|Grantee:||Fernando Campanhã Vicentini|
|Home Institution:||Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil|
New architectures for sensors and/or biosensors will be developed in this project. For this, glassy carbon electrodes and/or pyrolytic graphite electrodes will be modified with nanomaterials (carbon black, carbon nanotubes or metal nanoparticles) within chitosan, polyelectrolytes as polyallylamine hydrochloride or surfactants as dihexadecyl hydrogen phosphate films. For biosensors, enzymes of interest will be immobilized with glyoxal, glutaraldehyde, 1-ethyl-3-(3-dimethylaminopropyl) and carbodiimide/N-hydroxysuccinimide depending on the composition of the sensor base. The electrochemical characteristics of sensors/biosensors will be evaluated by electrochemical techniques such as: cyclic voltammetry, impedance spectroscopy and chronoamperometry. With these modifications, the objective is improve significantly the detection properties of these electrochemical devices, improving the electrochemical response in terms of the decrease in overpotential of the electrode, which is often high, reduce the interference of electroactive species, and may thus increase the selectivity. Furthermore, it is also expected to obtain an increase in the current magnitude (increase of the analytic signal) and/or increased sensitivity (slope of the calibration curve).The use of electrochemical probes such as potassium hexacyanoferrate(III) and/or hexaammineruthenium(III) will be employed to evaluate the electrochemical properties of electrodes modified with nanomaterials , being active area, the coverage area and heterogeneous electron transfer constant determined and compared. Initially will be evaluated the linear range of response, detection and quantification limits, sensitivity, potential interferences and lifetime of the electrodes. The electrodes with improved analytical performance will be applied in the determination of analytes of environmental, biological and pharmaceutical interest.