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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water

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Author(s):
Vicentini, Fernando Campanha [1, 2] ; Garcia, Livia L. C. [1] ; Figueiredo-Filho, Luiz C. S. [1, 3] ; Janegitz, Bruno C. [1, 4] ; Fatibello-Filho, Orlando [1]
Total Authors: 5
Affiliation:
[1] Univ Fed Sao Carlos, Dept Chem, Rod Washington Luis Km 235, POB 676, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Ctr Nat Sci, Rod Lauri Simoes Barros Km 12, BR-18290000 Buri, SP - Brazil
[3] Fed Inst Parana, Campus Paranavai, Rua Jose Felipe Tequinha 1400, BR-87703536 Paranavai, PR - Brazil
[4] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Enzyme and Microbial Technology; v. 84, p. 17-23, MAR 2016.
Web of Science Citations: 50
Abstract

In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5 x 10(-6) to 9.5 x 10(-6) mol L-1 with a detection limit of 1.7 x 10(-7) mol L-1. The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method. (C) 2015 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 13/16770-0 - Sensors development based on polymeric films and nanomaterials for several analytical applications
Grantee:Fernando Campanhã Vicentini
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/04284-6 - Electrochemical studies and application of sensors based on graphene or 3-D reticuled glassy carbon modified with metal nanoparticles for determination of pharmaceuticals and environmental analytes
Grantee:Luiz Carlos Soares de Figueiredo Filho
Support type: Scholarships in Brazil - Post-Doctorate