A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water

Autor(es)

Vicentini, Fernando Campanha ^{[1, 2]} ; Garcia, Livia L. C. ^[1] ; Figueiredo-Filho, Luiz C. S. ^{[1, 3]} ; Janegitz, Bruno C. ^{[1, 4]} ; Fatibello-Filho, Orlando ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem, Rod Washington Luis Km 235, POB 676, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Ctr Nat Sci, Rod Lauri Simoes Barros Km 12, BR-18290000 Buri, SP - Brazil

^[3] Fed Inst Parana, Campus Paranavai, Rua Jose Felipe Tequinha 1400, BR-87703536 Paranavai, PR - Brazil

^[4] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Enzyme and Microbial Technology; v. 84, p. 17-23, MAR 2016.
• Citações Web of Science: 45

Resumo

In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5 x 10(-6) to 9.5 x 10(-6) mol L-1 with a detection limit of 1.7 x 10(-7) mol L-1. The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 14/04284-6 - Estudos eletroquímicos e aplicação de sensores a base de grafeno ou carbono vítreo reticulado 3-D modificados com nanopartículas metálicas para a determinação de analitos de interesse farmacêutico e ambiental.
• Beneficiário: Luiz Carlos Soares de Figueiredo Filho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/16770-0 - Desenvolvimento de sensores à base de filmes poliméricos e nanomateriais para aplicações analíticas diversas
• Beneficiário: Fernando Campanhã Vicentini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado