Immobilization of dapsone in biomimetic systems: self-assembled, Langmuir and Langmuir-Blodgett films

• Grant number: 13/04246-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2013
• End date: May 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Osvaldo Novais de Oliveira Junior
• Grantee: Vananélia Pereira Nunes Geraldo
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM
• Associated scholarship(s): 14/03228-5 - Pegylated immunoliposomes containing paclitaxel: preparation, characterization, and antitumor activity, BE.EP.PD

Abstract

The first part of this project includes the fabrication and characterization of layer-by-layer films containing liposomes and the antibiotic dapsone. The liposomes will be made from two anionic phospholipids: dipalmitoyl phosphatidyl glycerol (DPPG) and dimyristoyl phosphatidic acid (DMPA). Drug-containing liposomes will be immobilized on nanostructured polymeric films via physical adsorption with the polyamidoamine dendrimer (PAMAM). Interactions between liposomes and dapsone will be studied with emphasis on immobilization of these materials in LbL films and the antibiotic release from these systems in vitro. These interactions will be analysed by dynamic light scattering (DLS), Fourier transform infrared (FTIR), fluorescence and UV-vis. spectroscopies. The structural and morphological characteristics of the films will be evaluated using atomic force microscopy (AFM), quartz crystal microbalance (QCM) and polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS). In the second part of this project, to be performed abroad, we intend to investigate the interaction of dapsone with DPPG and DMPA monolayers by Langmuir and Langmuir-Blodgett techniques. These interactions will be analyzed by changing parameters such as spreading method, drug concentration and pH. Langmuir films will be characterized using surface pressure and surface potential isotherms, in addition to Brewster angle microscopy (BAM). For LB film characterization we shall use AFM, fluorescence and UV-vis spectroscopies. The effect from dapsone on the thermotropic behavior of the lipid systems will be investigated using differential scanning calorimetry (DSC).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GERALDO, VANANELIA P. N.; ZIGLIO, ANALINE C.; GONCALVES, DEBORA; OLIVEIRA, JR., OSVALDO N.. Interaction of capsaicinoids with cell membrane models does not correlate with pungency of peppers. Chemical Physics Letters, v. 673, p. 78-83, APR 2017. (13/14262-7, 13/04246-4)