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Molecular interaction between chitosan and cell membrane models investigated by non-linear vibrational spectroscopy

Grant number: 17/03503-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): August 01, 2017
Effective date (End): March 11, 2020
Field of knowledge:Physical Sciences and Mathematics - Physics - Condensed Matter Physics
Principal researcher:Paulo Barbeitas Miranda
Grantee:Rafael de Oliveira Pedro
Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated research grant:13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM

Abstract

Chitosan has been used in many biological applications such as drug delivery, gene therapy, tissue engineering and antimicrobial systems. Although these applications have grown greatly in recent years, little is known about the modes of interaction at the molecular level of chitosan with biological membranes. Therefore, interactions between chitosan and membrane models of mammals, bacteria and fungi will be investigated in this work. Firstly, biologically relevant temperature will be implemented in the experiments in order to allow the extrapolation of the results obtained for in vitro or in vivo situations. Following, Sum-Frequency Generation spectroscopy (SFG) will provide information about the interaction of chitosan with the polar group of lipids, conformation of the lipid chains and the penetration of chitosan in the membrane models. Sum-Frequency Generation microscopy (MSFG microscopy), an innovative technique, will be used to obtain images at interfaces with chemical sensitivity by the vibrational spectrum and contrast by the orientation and ordering of the molecules. The techniques of polarization-modulated infrared spectroscopy (PM-IRRAS) and Brewster angle microscopy (BAM) will be used for comparison with results from SFG spectroscopy and microscopy. The results are expected to provide a solid basis for understanding the role of positive charges, degree of hydrophobicity, and hydrogen bonds in the interactions between chitosan and membrane models. Therefore, this work will contribute to elucidate the mechanism of molecular interaction of chitosan derivatives with cellular membranes of mammals, bacteria and fungi, using powerful techniques, although not widely used in this area.

News published in Agência FAPESP Newsletter about the scholarship:
Un estudio puede ayudar a desarrollar biosensores para el diagnóstico no invasivo de enfermedades 
Study could help develop biosensors for non-invasive diagnosis of diseases 
Estudo pode ajudar a desenvolver biossensores para diagnóstico não invasivo de doenças 
Articles published in other media outlets (29 total):
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Global Health News Wire (EUA): Study Could Help Develop Biosensors for Non-Invasive Diagnosis of Diseases (24/Mar/2021)
Medica Trade Fair (Alemanha): Biosensors help with non-invasive diagnosis of diseases (24/Mar/2021)
News Medical (Austrália): Study may help develop effective biosensors for non-invasive diagnosis of prostate cancer (23/Mar/2021)
AZoSensors (Reino Unido): Brazilian Researchers Test the Capacity of Different Materials Used in Biosensors (23/Mar/2021)
Global Biodefense (EUA): Technique for Testing Biosensor Materials Could Aid Noninvasive Diagnostic Development (23/Mar/2021)
Freeschi: Study may help develop effective biosensors for non-invasive diagnosis of prostate cancer (23/Mar/2021)
The Uncover Reality (Índia): Study Could Help Develop Biosensors For Non-invasive Diagnosis of Diseases (Material Science) (23/Mar/2021)
SciFi News Hubb: Study could help develop biosensors for non-invasive diagnosis of diseases (23/Mar/2021)
Medical Xpress (Reino Unido): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
Bioengineer (Reino Unido): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
Scienmag Science Magazine (Reino Unido): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
7thSpace: Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
About Cancer Service: Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
Corona Stocks (Canadá): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
Vighneshworld.com: Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
Zephyrnet.com (EUA): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
Scifi insight: Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
SciFi Voice (EUA): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
Biotech Asia News (Índia): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
MediExpose (ìndia): Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
My Best Medicine: Home » Health News » Study could help develop biosensors for non-invasive diagnosis of diseases (22/Mar/2021)
LabNetwork: Estudo pode ajudar a desenvolver biossensores para diagnóstico não invasivo de doenças (17/Feb/2021)
Biblioteca FMUSP: Estudo pode ajudar a desenvolver biossensores para diagnóstico não invasivo de doenças (16/Feb/2021)
Saense: Estudo pode ajudar a desenvolver biossensores para diagnóstico não invasivo de doenças (16/Feb/2021)
Diário da Saúde: Biossensores ajudarão em diagnóstico não invasivo de doenças (15/Feb/2021)
LIM - Laboratórios de Investigação Médica: Estudo pode ajudar a desenvolver biossensores para diagnóstico não invasivo de doenças (15/Feb/2021)
Programa InfoSalud (Argentina): Un estudio puede ayudar a desarrollar biosensores para diagnóstico no invasivo de enfermedades (15/Feb/2021)
FOCEP Brasil: Estudo pode ajudar a desenvolver biossensores para diagnóstico não invasivo de doenças (15/Feb/2021)
Brasil Amazônia Agora: Estudo pode ajudar a desenvolver biossensores para diagnóstico não invasivo de doenças (15/Feb/2021)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RAYMUNDO-PEREIRA, PAULO A.; PEDRO, RAFAEL DE OLIVEIRA; CARR, OLIVIA; MELENDEZ, MATIAS E.; GOBBI, ANGELO L.; PIAZZETTA, MARIA HELENA DE OLIVEIRA; CARVALHO, ANDRE L.; REIS, RUI M.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N. Influence of the Molecular Orientation and Ionization of Self-Assembled Monolayers in Biosensors: Application to Genosensors of Prostate Cancer Antigen 3. Journal of Physical Chemistry C, v. 125, n. 1, p. 498-506, JAN 14 2021. Web of Science Citations: 0.
PEREIRA, ANDRESSA R.; FIAMINGO, ANDERSON; PEDRO, RAFAEL DE O.; CAMPANA-FILHO, SERGIO P.; MIRANDA, PAULO B.; OLIVEIRA JR, OSVALDO N. Enhanced chitosan effects on cell membrane models made with lipid raft monolayers. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 193, SEP 2020. Web of Science Citations: 0.
PEDRO, RAFAEL DE OLIVEIRA; PEREIRA, ANDRESSA RIBEIRO; OLIVEIRA, OSVALDO N.; MIRANDA, PAULO BARBEITAS. Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 192, AUG 2020. Web of Science Citations: 1.
CARR, OLIVIA; RAYMUNDO-PEREIRA, PAULO A.; SHIMIZU, FLAVIO M.; SORROCHE, BRUNA PEREIRA; MELENDEZ, MATIAS ELISEO; PEDRO, RAFAEL DE OLIVEIRA; MIRANDA, PAULO B.; CARVALHO, ANDRE LOPES; REIS, RUI M.; ARANTES, LIDIA M. R. B.; OLIVEIRA, JR., OSVALDO N. Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines. Talanta, v. 210, APR 1 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.