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Photodynamic therapy: from molecular effects in model systems toward photodynamic efficiency in cell culture


This research grant has the general objective of continuing the work of the past Fapesp (2016 / 13280-0) research project, as well as to support the research of the graduate students under my supervision. The project is focused on the photosensitization of lipid membranes and underlying mechanisms of the photodynamic efficiency in the treatment of cancer cells. Specifically, Langmuir and Langmuir-Blodgett (LB) films will mimic the cell membrane of tumor cells, where photosensitizers will be further incorporated and subsequently photo-activated. The goal is to investigate the effects of lipid hydroperoxidation on the physicochemical properties of the membrane, which include surface area increase and cleavage of lipid chains. In addition to the traditional surface pressure measurements (À), vibrational spectroscopy techniques will be applied to the Langmuir (PM-IRRAS) and LB (FTIR) films in order to elucidate the reaction mechanisms at the molecular level. The final goal is to correlate the findings at the molecular scale with photodynamic efficiency in complex systems, involving the in vitro culture of cancer cells. Taken together, these results will shed light on the molecular mechanisms of cell membrane photo-oxidation, which is relevant not only for photodynamic therapy (PDT) applications but also for many other fields of life sciences and health. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PAZIN, WALLANCE MOREIRA; MARQUES RUIZ, GILIA CRISTINE; DOS SANTOS, MARCELO JOSE; BENITES AOKI, PEDRO HENRIQUE; ITO, AMANDO SIUITI; LEOPOLDO CONSTANTINO, CARLOS JOSE. The protective effect of Artepillin C against lipid oxidation on model membranes. JOURNAL OF MOLECULAR LIQUIDS, v. 324, . (16/09633-4, 18/22214-6, 18/16713-0)
MOREIRA, LUCAS G.; ALMEIDA JR, ALEXANDRE M.; NIELD, TYLER; CAMACHO, SABRINA A.; AOKI, PEDRO H. B.. Modulating photochemical reactions in Langmuir monolayers of Escherichia coli lipid extract with the binding mechanisms of eosin decyl ester and toluidine blue-O photosensitizers. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 218, . (20/15854-9, 18/16713-0, 18/22214-6, 18/14692-5)
MOREIRA, LUCAS G.; ALMEIDA JR, ALEXANDRE M.; CAMACHO, SABRINA A.; ESTEVAO, BIANCA M.; OLIVEIRA JR, OSVALDO N.; AOKI, PEDRO H. B.. Chain Cleavage of Bioinspired Bacterial Membranes Photoinduced by Eosin Decyl Ester. Langmuir, v. 36, n. 32, p. 9578-9585, . (18/22214-6, 18/14692-5, 13/14262-7, 18/13021-0, 18/16713-0)
ALMEIDA, JR., ALEXANDRE M.; OLIVEIRA, JR., OSVALDO N.; AOKI, PEDRO H. B.. Role of Toluidine Blue-O Binding Mechanism for Photooxidation in Bioinspired Bacterial Membranes. Langmuir, v. 35, n. 51, p. 16745-16751, . (13/14262-7, 18/16713-0)
CAMACHO, SABRINA A.; KOBAL, MIRELLA B.; MOREIRA, LUCAS G.; BISTAFFA, MARIA J.; ROQUE, THAMIRES C.; PAZIN, WALLANCE M.; TOLEDO, KARINA A.; OLIVEIRA JR, OSVALDO N.; AOKI, PEDRO H. B.. The efficiency of photothermal action of gold shell-isolated nanoparticles against tumor cells depends on membrane interactions. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 211, . (18/14692-5, 18/16713-0, 20/12129-1, 20/15854-9, 14/11408-3, 17/03879-4, 18/22214-6)
BISTAFFA, MARIA J.; KOBAL, MIRELLA B.; SOUZA, PRISCILA S. S.; TOLEDO, KARINA A.; CAMACHO, SABRINA A.; AOKI, PEDRO H. B.. Photo-Induced Necrosis on Oropharyngeal Carcinoma (HEp-2) Cells Mediated by the Xanthene Erythrosine. Journal of Nanoscience and Nanotechnology, v. 20, n. 10, p. 6180-6190, . (13/14262-7, 16/20576-2, 18/14692-5, 18/16713-0)
BISTAFFA, MARIA J.; CAMACHO, SABRINA A.; MELO, CARLOS F. O. R.; CATHARINO, RODRIGO R.; TOLEDO, KARINA A.; AOKI, PEDRO H. B.. Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 223, . (18/14692-5, 13/14262-7, 16/20576-2, 18/16713-0)

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