Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium

Full text
Pedro, Rafael de Oliveira [1] ; Pereira, Andressa Ribeiro [1] ; Oliveira, Osvaldo N. [1] ; Miranda, Paulo Barbeitas [1]
Total Authors: 4
[1] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Web of Science Citations: 1

Hypothesis: The interaction of chitosan, a natural biopolymer with various biomedical applications, with lipid Langmuir films has been widely investigated as a simple model for cell membranes. However, to ensure polymer solubility, up to now only acidic subphases with pH significantly below biological fluids have been used. To increase the biological significance of these investigations, here we evaluated the effects of two chitosan derivatives (low molecular weight - CH, and positively charged - CH-P-40) on phospholipid films (either zwitterionic DPPC or anionic DPPG) using phosphate buffered saline solutions (PBS) as a subphase. Experiments: Surface pressure - area (pi - A) isotherms were used to evaluate the expansion and changes in film elasticity, while Sum-Frequency Generation (SFG) vibrational spectroscopy provided information about the chain conformation of lipids. Findings: It was found that chitosans caused a small expansion of the DPPC film by its insertion within the monolayer. In contrast, they distinctly expanded DPPG monolayers by both chitosan insertion within the lipid monolayer and by interacting with the anionic head group. Therefore, PBS buffer can be used as a subphase for more biologically relevant studies of chitosan interactions with Langmuir films, shedding light on why chitosan is antibacterial but not toxic to mammals, as the interaction mechanism depends on lipid headgroup charge. (AU)

FAPESP's process: 18/00878-0 - Study of lipid rafts through the interaction between membrane models and chitosan and cholesterol oxidase
Grantee:Andressa Ribeiro Pereira
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/02819-0 - Interaction of chitosans and surfactants with cell membrane models investigated by nonlinear vibrational spectroscopy
Grantee:Paulo Barbeitas Miranda
Support Opportunities: Regular Research Grants
FAPESP's process: 17/03503-4 - Molecular interaction between chitosan and cell membrane models investigated by non-linear vibrational spectroscopy
Grantee:Rafael de Oliveira Pedro
Support Opportunities: Scholarships in Brazil - Post-Doctoral