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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interaction of chitosan derivatives with cell membrane models in a biologically relevant medium

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Autor(es):
Pedro, Rafael de Oliveira [1] ; Pereira, Andressa Ribeiro [1] ; Oliveira, Osvaldo N. [1] ; Miranda, Paulo Barbeitas [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 192, AUG 2020.
Citações Web of Science: 1
Resumo

Hypothesis: The interaction of chitosan, a natural biopolymer with various biomedical applications, with lipid Langmuir films has been widely investigated as a simple model for cell membranes. However, to ensure polymer solubility, up to now only acidic subphases with pH significantly below biological fluids have been used. To increase the biological significance of these investigations, here we evaluated the effects of two chitosan derivatives (low molecular weight - CH, and positively charged - CH-P-40) on phospholipid films (either zwitterionic DPPC or anionic DPPG) using phosphate buffered saline solutions (PBS) as a subphase. Experiments: Surface pressure - area (pi - A) isotherms were used to evaluate the expansion and changes in film elasticity, while Sum-Frequency Generation (SFG) vibrational spectroscopy provided information about the chain conformation of lipids. Findings: It was found that chitosans caused a small expansion of the DPPC film by its insertion within the monolayer. In contrast, they distinctly expanded DPPG monolayers by both chitosan insertion within the lipid monolayer and by interacting with the anionic head group. Therefore, PBS buffer can be used as a subphase for more biologically relevant studies of chitosan interactions with Langmuir films, shedding light on why chitosan is antibacterial but not toxic to mammals, as the interaction mechanism depends on lipid headgroup charge. (AU)

Processo FAPESP: 17/03503-4 - Interação molecular entre quitosanas e modelos de membrana celular investigada por espectroscopia vibracional não linear
Beneficiário:Rafael de Oliveira Pedro
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/00878-0 - Estudo de jangadas lipídicas com a interação entre modelos de membrana e quitosana e colesterol oxidase
Beneficiário:Andressa Ribeiro Pereira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/02819-0 - Interação de quitosanas e surfactantes com modelos de membrana celular investigada por espectroscopia vibracional não linear
Beneficiário:Paulo Barbeitas Miranda
Linha de fomento: Auxílio à Pesquisa - Regular