Immunoreactivity of proteins within 30-40 kDa range during the acute and the recovery phases in rats experimentally infected with Strongyloides venezuelensis

Autor(es)

Priscilla Duarte Marques Fonseca ^[1] ; Marcelo Andreeta Corral ^[2] ; Dirce Mary C. Lima Meisel ^[3] ; Debora Levi ^[4] ; Rafael Correa Nascimento ^[5] ; William Castro-Borges ^[6] ; Ronaldo Gryschek ; Julia Maria Costa-Cruz ^[8] ; Fabiana Martins de Paula

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo - Brasil

^[2] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

^[3] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

^[4] Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas - Brasil

^[5] Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo - Brasil

^[6] Universidade Federal de Ouro Preto. Departamento de Ciências Biológicas - Brasil

^[8] Universidade Federal de Uberlândia. Laboratório de Diagnóstico de Parasitoses - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 62, 2020-05-11.

Resumo

ABSTRACT In experimental infection with Strongyloides venezuelensis, the acute and recovery phases can be distinguished, unlike human infections caused by Strongyloides stercoralis. The objective of this study was to evaluate the production of anti-Strongyloides IgG antibodies and the recognition of immunogenic protein bands during the acute and the recovery phases in rats experimentally infected with S. venezuelensis. Rats were infected subcutaneously with 400 or 4,000 S. venezuelensis infective larvae. The acute phase was characterized by elimination of a large number of eggs in the faeces on days 6-14 post infection; the recovery phase was characterized by the resolution of the infection between days 30 and 35 post infection. Differences in IgG levels were observed in the acute and the recovery phases. Different antigenic fractions were recognized in both phases of infection. It is concluded that proteins within the 30-40 kDa range are immunoreactive markers for both the acute and the recovery phases in rats experimentally infected with S. venezuelensis, particularly using membrane antigen. (AU)

• Processo FAPESP: 16/06185-0 - Análise proteômica de diferentes estágios evolutivos de Strongyloides venezuelensis
• Beneficiário: Fabiana Martins de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Regular